Room Temperature Storage and Shipping of Purified RNA

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As generating expression profiles with next generation sequencing becomes more popular the need for a reliable method to transport purified RNA is paramount. It is increasingly common for collaboration among multiple sites where RNA is purified at one location and analyzed at another. Current methods include overnight shipments on dry ice or drying samples into a gel matrix.

Transporting purified RNA offers unique challenges as the sample can degrade during shipment, in preparation for shipment, in transit or during the receiving process. During transit, samples can also degrade due to carrier delays, samples becoming displaced from the dry ice or dry ice evaporation. With these conditions in mind, Omega Bio-tek has developed RNA Transport to eliminate dry ice shipments allowing for samples to be shipped via ground transportation at significant cost savings while maintaining RNA quality.

Effects of Room Temperature Storage with RNA Transport


Total RNA was purified from cultured cells using the E.Z.N.A.® Total RNA Kit 1. 4 µg was aliquoted to individual tubes containing RNA Transport then stored at room temperature for the indicated days. RNA was re-purified using RNA Transport's purification method which takes less than 5 minutes per sample set. RNA quality was analyzed via Agilent TapeStation 2200.

To stabilize purified RNA, samples simply need to be mixed with RNA Transport. No long, tedious lyphilization steps or creating a gel matrix; in less than 10 seconds your RNA sample is protected. Once received, RNA samples can be left at room temperature until the receiving site is ready to process them, thus eliminating the need for -80°C storage.

Save on Shipping Costs

RNA Transport eliminates the need for bulk boxes, dry ice and styrofoam containers and overnight shipping. The bulky packaging requirements for dry ice increases shipping charges and usually require an overnight service or, with international shipments, that dry ice can be replenished. RNA Transport is supplied in 12 mm, screw cap, 2.0 mL vials for ease of use and reduction of shipping costs.

Cost analysis of shipping dry ice packages overnight versus shipping in smaller packages via ground shipments.

Automation

For core facilities and sequencing centers, a common problem is receiving samples in various plasticware which is not compatible with automation or does not match existing labware definitions. Having customers who use RNA Transport allows for your procedures to be standardized and sample transfer to be automated with liquid handling instruments with individual probes such as Hamilton STAR/STARlet, Tecan Evo and Beckman Coulter Biomek FX/NX.

Flexibility

RNA Transport allows customers to ship samples any day of the week instead of limiting shipments to Monday-Wednesday. Even in extreme temperatures during winter and summer months, RNA Transport is able to protect samples for use in downstream applications.

International customers have the added security that if samples are delayed in customs, the RNA will still be safe and intact.

Variable Temperatures

Sample RIN Value
Original sample 9.4
2x Freeze Thaw A 9.1
2x Freeze Thaw B 9.0
37°C for 7 days A 8.4
37°C for 7 days B 8.5

Variable Temperatures. Total RNA was purified from cultured cells using the E.Z.N.A.® Total RNA Kit 1. 4 µg RNA was aliquoted to individual tubes containing RNA Transport then stored at -20°C for 24 hours, thawed at room temperature for 24 hours for the indicated days. RNA was re-purified using RNA Transport's purification method, which takes less than 5 minutes per sample set. RNA quality was analyzed via Agilent TapeStation 2200.

Ordering Information

Product No Description Size
R0527-00 RNA Transport 5 Preps
R0527-01 RNA Transport 50 Preps
R6834-01 E.Z.N.A.® Total RNA Kit 1 50 Preps
R6834-02 E.Z.N.A.® Total RNA Kit 1 200 Preps
RNACOL-01 HiBind RNA Mini Columns 50 Preps
RNACOL-02 HiBind RNA Mini Columns 200 Preps