The Firefly NIMBUS 96 workstation is a compact, automated liquid handler specifically configured for utilizing Omega Bio-tek's Mag-Bind® chemistry for nucleic acid purification. The system offers speed, flexibility, ease-of-use, and superior pipetting performance at a surprisingly affordable price. It incorporates predefined methods for processing a wide variety of sample types and is backed by Omega Bio-tek's and Hamilton's renowned service and applications support.
Omega Bio-tek offers highly versatile DNA and RNA extraction kits covering a wide spectrum of sample types, employing either proprietary silica membrane-based or magnetic bead-based technologies, suitable for both manual and automated extractions. Our Mag-Bind® technology provides a flexible and reliable method for isolating nucleic acids using automated liquid handlers.
|Sample Type||Target Nucleic Acid||Product||Amount||Offline/Online Processing Time|
|Whole blood, saliva||gDNA||Mag-Bind® Blood & Tissue DNA HDQ 96 Kit||10-250 µL||5 min/80 min|
|Buccal swabs||gDNA||Mag-Bind® Blood & Tissue DNA HDQ 96 Kit||1 swab||30 min/70 min|
|Serum/plasma||Cell-free DNA||Mag-Bind® cfDNA Kit||750 µL||5 min/80 min|
|Bacterial, fungal, yeast DNA, viral DNA/RNA||Mag-Bind® Universal Pathogen DNA 96 Kit||10-250 µL||30 min/60 min|
|Viral DNA & RNA||Mag-Bind® Viral DNA/RNA Kit||10-200 µL||5 min/60 min|
|Cultured cells & soft tissues||gDNA||Mag-Bind® Blood & Tissue DNA HDQ 96 Kit||1x106 or
5-10 mg tissue
|5 min/80 min
1-3 hrs/70 min
|Total RNA||Mag-Bind® Total RNA 96 Kit||1x106 or
5-10 mg tissue
|20 min/70 min|
|FFPE||gDNA||Mag-Bind® FFPE DNA 96 Kit||3-5 sections||3-5 hrs/70 min|
|Urine||Bacterial, fungal, yeast DNA, viral DNA & RNA||Mag-Bind® Universal Pathogen DNA 96 Kit||10-250 µL||30 min/60 min|
|Soil & environmental samples||Bacterial, yeast, fungal DNA||Mag-Bind® Environmental DNA 96 Kit||250 mg||45 min/60 min|
|PCR products & next-gen sequencing||Fragmented DNA||Mag-Bind® TotalPure NGS||10-50 µL||5 min/30 min|
|Fecal||Bacterial, fungal, yeast DNA, viral DNA & RNA||Mag-Bind® Universal Pathogen DNA 96 Kit||10-250 µL||30 min/60 min|
|Plant tissue||gDNA||Mag-Bind® Plant DNA DS 96 Kit||50-100 mg||50 min/85 min|
Hamilton utilizes proven air displacement pipetting, similar to high precision handheld electronic pipettors.
Compressed O-ring expansion (CO-RE) technology attaches disposable tips using a highly robust lock-and-key style mechanism.
Capacitative LLC (c-LLD) can determine liquid levels in plates and reservoirs located on the pipetting deck.
Each predefined script can process between 1-96 samples per run. The final elution volume for each method can be set between 50-200 µL, providing flexibility to the end user for adjusting final DNA or RNA concentrations.
The number of samples entered in step 1 will determine how many racks of tips will be required to load onto the system deck. Each method uses the absolute minimum amount of tips to process the number of samples entered by the user to conserve tips and reduce consumable costs.
Four-column reservoirs are used for the methods to maximize deck space. This prompt depicts the amount and location of reagent relative to the individual reservoir. The volume for each buffer is calculated based on the number of samples being processed.
This prompt provides a final overview of the deck layout and instructs the user on where to load all other components, including the starting plate, reagent reservoirs, water rinse plate, and final elution plate.
DNA and RNA can be purified with minimal hands-on requirements and can easily be adapted for full or partial plate processing. Optimized programming gives you an out-of-the-box solution that allows for successful implementation of your downstream application.
Wheter you are performing next-generation sequencing on clinical samples or metagenomics from rare environmental samples, Omega Bio-tek offers a chemistry to fit your evolving application needs.
The Mag-Bind® Blood & Tissue DNA HDQ 96 Kit is designed for high quality genomic DNA purification from a wide variety of sample types. Predefined Firefly NIMBUS 96 methods are available for using this kit to process whole blood, buccal swabs, tail snips, dried blood spots, tissue, and even cultured cells. E-Z 96™ Spin-Out Baskets can be used in conjunction with this kit to provide a fast and convenient way to process buccal swabs and dried blood spots in a high throughput fashion.
Average yield from buccal swabs extracted using Mag-Bind® technology on the Firefly NIMBUS 96, BioSprint® 96, and manually.
|Whole blood||250 µL||6.4|
|Cultured cells (HEK293)||1x106||10.6|
Average yield from different sample types processed on the Firefly NIMBUS 96.
The Mag-Bind® Universal Pathogen DNA 96 Kit enables purification of high quality DNA from bacteria (Gram positive and Gram negative), fungal spores, and viral DNA/RNA, along with host genomic DNA from a whole range of samples such as tissues, urine, and feces.
|Extraction Method||Average Ct|
|Firefly NIMBUS 96||20.6||23.8||27.1|
Real-time PCR with Gram-positive specific bacterial primers performed in triplicate at 1X, 10X, and 100X dilutions of DNA isolated using three different extraction methods.
Average yield from 250 µL stool samples spiked in with 20 µL ZymoBIOMICS™ Microbial Community Standard (Zymo Research). (n=3) extracted on the Firefly NIMBUS 96, Qiagen BioSprint® 96, and manually. (n=3).
The Mag-Bind® Plant DNA DS 96 Kit allows rapid and reliable isolation of high quality genomic DNA from a variety of plant species containing high amounts of polysaccharides and polyphenols. The system combines the robust lysing power of cationic detergent, cetyltrimethylammonium bromide (CTAB) with the rapid response of the Mag-Bind® particles to efficiently purify high quality DNA.
Genomic DNA from canola leaf sample extracted manually and on Firefly NIMBUS 96 analyzed on Agilent's TapeStation® 2200.