Genomic DNA

Q: What is the composition of Elution Buffer?
A: 10 mM Tris Hcl pH 8.5

Q: Can the Tissue DNA Kit be used for whole blood samples?
A: Yes, download the Blood DNA Kit (D3392) protocol and follow accordingly.

Q: Can the Bacterial DNA Kit isolate gram positive and negative species?
A:Yes, the Bacterial DNA Kit uses glass beads and lysozyme to remove the cell wall.

Q: What is the advantage of MicroElute columns?
A: MicroElute columns allow for elution volumes as small as 10 mL. They are not recommended for samples when more than 12 µg of DNA is being recovered.

Q: What kit can be used for paraffin-embedded tissues?
A: The Tissue DNA Kit can be used for FFPE tissues.

Q: Can centrifugation method be used with magnetic bead-based kits?
A: Yes, centrifugation steps can be substituted for magnetizing steps.

Q: What is the difference between the SQ I Blood DNA Kit and the SQ II Blood DNA Kit?
A: The SQ I Blood DNA Kit first selectively lyses red blood cells and then lyses white blood cells. The kit then uses buffer to precipitate out the proteins. The SQ II Blood DNA Kit first lyses the red and white blood cells down to the cell nucleus. The nucleus is then lysed and the proteins are digested with proteinase K. Both kits then require an isopropanol precipitation.

Q: Will the Blood DNA Kit isolate mitochondrial DNA?
A: Yes, mitochondrial and viral DNA present will be isolated.

Q: Can you use a vacuum protocol with the E-Z 96® Blood DNA Kit with whole blood?
A: For whole blood, use the E-Z 96® HiFlow Blood DNA Kit. Clogging may occur with the E-Z 96® Blood DNA Kit.

Q: What kit can clean up crude genomic DNA lysates?
A: The Blood DNA Kit can be used for genomic DNA clean-up. Substitute the lysate in for whole blood and follow the protocol accordingly. If the sample contains humic acid, the modified protocol for clean-up of DNA samples from the Stool DNA Kit is recommended.

DNA/RNA Cleanup

Q: What is the difference between Q-spin and V-spin columns?
A: V-spin columns have an attached cap while Q-spin columns are capless.

Q: What is the composition of ELution Buffer?
A: 10 mM Tris Hcl pH 8.5

Q: Can the Gel Extraction Kit be used for clean-up of DNA from enzymatic reactions?
A: Yes, follow the protocol included with the booklet.

Q: Can the Cycle Pure Kit be used for Gel Extractions?
A: Unfortunately not. Please use the Gel Extraction Kit.

Q: What is the difference between the Ultra Sep Gel Extraction Kit and the Gel Extraction Kit?
A: The Ultra Sep Gel Extraction Kit uses silica beads to bind the DNA while the Gel Extraction Kit uses a silica column. The silica beads need to be pelleted and resuspended for binding and washing steps while the Gel Extraction Kit uses a silica column which the liquid flows through.

Plasmid DNA

Q: What is the difference between Q-spin and V-spin columns?
A: V-spin columns have an attached cap while Q-spin columns are capless.

Q: What is the difference between Plasmid Mini Kit I and Plasmid Mini Kit II?
A: Plasmid Mini Kit I is designed for culture volumes up to 5 mL while the Plasmid Mini Kit II is designed for culture volumes between 10-15 mL. Both kits can be processed in a microcentrifuge or vacuum manifold.

Q: What is the composition of Elution Buffer?
A: 10 mM Tris Hcl pH 8.5

Q: What is the composition of Solution I?
A: 50 mM NaOH, 1% SDS

Q: What is the composition of Solution II?
A: 200 mM NaOH, 1% SDS

Q: What is the largest size plasmid that can be isolated with the Plasmid Mini Kit I?
A: Tests have shown that plasmids up to 57 kb can be isolated. No modified protocol is needed; expect to warm Elution Buffer to 70° Celsius and incubate for 2 minutes.

Q: What company’s vacuum manifolds are compatible with the E-Z 96® FastFilter Plasmid Mini Kit?
A: Qiagen’s QIAvac 96 and Macherey Nagel’s NucleoVac 96.

Q: Are the spin columns sold separately?
A: Yes, the product number is DNACOL-02.