Hamilton Firefly NIMBUS

The Firefly NIMBUS 96 workstation is a compact, automated liquid handler specifically configured for utilizing Omega Bio-tek’s Mag-Bind® chemistry for nucleic acid purification. The system offers speed, flexibility, ease of use, and superior pipetting performance at a surprisingly affordable price. It incorporates predefined methods for processing a wide variety of sample types and is backed by Omega Bio-tek’s and Hamilton’s renowned service and applications support.

Hamilton Firefly NIMBUS
High quality, economical, and diverse product range for DNA and RNA purification customized to your requirements

Omega Bio-tek offers highly versatile DNA and RNA extraction kits covering a wide spectrum of sample types, employing either proprietary silica membrane-based or magnetic bead-based technologies, suitable for both manual and automated extractions. Our Mag-Bind® technology provides a flexible and reliable method for isolating nucleic acids using automated liquid handlers.

Instrument Configuration

 

Applications
Sample type Target nucleic acid Product Amount Offline/online processing time
Whole blood, saliva gDNA Mag-Bind® Blood & Tissue DNA HDQ 96 Kit 10-250 µL 5 min/80 min
Buccal swabs gDNA Mag-Bind® Blood & Tissue DNA HDQ 96 Kit 1 swab 30 min/70 min
Serum/plasma Cell-free DNA Mag-Bind® cfDNA Kit 750 µL 5 min/80 min
Bacterial, fungal, yeast DNA, viral DNA/RNA Mag-Bind® Universal Pathogen Kit 10-250 µL 30 min/60 min
Viral DNA & RNA Mag-Bind® Viral DNA/RNA Kit 10-200 µL 5 min/60 min
Cultured cells & soft tissues gDNA Mag-Bind® Blood & Tissue DNA HDQ 96 Kit 1×106 or 5-10 mg tissue 5 min/80 min
1-3 hrs/70 min
Total RNA Mag-Bind® Total RNA Kit 1×106 or 5-10 mg tissue 20 min/70 min
FFPE gDNA Mag-Bind® FFPE DNA Kit 3-5 sections 3-5 hrs/70 min
Urine Bacterial, fungal, yeast DNA, viral DNA & RNA Mag-Bind® Universal Pathogen Kit 10-250 µL 30 min/60 min
Soil & environmental samples Bacterial, yeast, fungal DNA Mag-Bind® Environmental DNA Kit 250 mg 45 min/60 min
PCR products & next-generation sequencing Fragmented DNA Mag-Bind® TotalPure NGS 10-50 µL 5 min/30 min
Fecal Bacterial, fungal, yeast DNA, viral DNA & RNA Mag-Bind® Universal Pathogen Kit 10-250 µL 30 min/60 min
Plant tissue gDNA Mag-Bind® Plant DNA DS Kit 50-100 mg 50 min/85 min

 

Unique Features
Air Displacement Pipetting

Hamilton utilizes proven air displacement pipetting, similar to high precision handheld electronic pipettors.

Compressed O-Ring Expansion

Compressed O-ring expansion attaches disposable tips using a highly robust lock-and-key style mechanism.

Liquid Level Detection

Capacitative LLD (c-LLD) can determine liquid levels in plates and reservoirs located on the pipetting deck.

Software & Setup
Step 1. Input sample number & elution volume

Each predefined script can process between 1-96 samples per run. The final elution volume for each method can be set between 50-200 µL, providing flexibility to the end user for adjusting final DNA or RNA concentrations.

Step 2. Load tips & tip isolator plate

The number of samples entered in step 1 will determine how many racks of tips will be required to load onto the system deck. Each method uses the absolute minimum amount of tips to process the number of samples entered by the user to conserve tips and reduce consumable costs.

Step 3: Set up buffers in reagent reservoirs

Four-column reservoirs are used for the methods to maximize deck space. This prompt depicts the amount and location of reagents relative to the individual reservoir. The volume for each buffer is calculated based on the number of samples being processed.

Step 4: Final checklist for loading all components

This prompt provides a final overview of the deck layout and instructs the user on where to load all other components, including the starting plate, reagent reservoirs, water rinse plate, and final elution plate.

Unmatched Flexibility

DNA & RNA can be purified with minimal hands-on requirements and can easily be adapted for full or partial plate processing. Optimized programming gives you an out-of-the-box solution that allows for successful implementation of your downstream application.

Whether you are performing next-generation sequencing on clinical samples or metagenomics from rare environmental samples, Omega Bio-tek offers a chemistry to fit your evolving application needs.

Mag-Bind® Blood & Tissue DNA HDQ 96 Kit

The Mag-Bind® Blood & Tissue DNA HDQ 96 Kit is designed for high quality genomic DNA purification from a wide variety of sample types. Predefined Firefly NIMBUS 96 methods are available for using this kit to process whole blood, buccal swabs, tail snips, dried blood spots, tissue, and even cultured cells. E-Z 96® Spin-Out Baskets can be used in conjunction with this kit to provide a fast and convenient way to process buccal swabs and dried blood spots in a high throughput fashion.

DNA Yield from Buccal Swabs

Average yield from buccal swabs extracted using Mag-Bind® technology on the Firefly NIMBUS 96, BioSprint 96, and manually.

Sample Amount Yield (µg)
Saliva 250 µL 2.0
Whole blood 250 µL 6.4
Cultured cells (HEK293) 1 x 106 10.6

Average yield from different sample types processed on the Firefly NIMBUS 96.

Sample Types Processed

Cultured cells Whole blood
Buccal swabs Mouse tails
Tissues
Mag-Bind® Universal Pathogen Kit
The Mag-Bind® Universal Pathogen DNA Kit enables purification of high quality DNA from bacteria (Gram positive and Gram negative), fungal spores, and viral DNA/RNA, along with host genomic DNA from a whole range of samples such as tissues, urine, and feces.

  • Inhibitor-free DNA acceptable for various downstream applications
  • Ideal for downstream pathogen detection testing
  • Pre-filled glass beads in a 96-well Disruptor Plate

 

Extraction Method Average Ct
1X 10X 100X
Firefly NIMBUS 96 20.6 23.8 27.1
BioSprint 96 20.9 24.0 27.6
Manual extraction 20.6 23.8 27.3

Real-time PCR with Gram positive-specific bacterial primers performed in triplicate 1X, 10X, and 100X dilutions of DNA isolated using three different extraction methods.

DNA Yield from Fecal Samples

Average yield from 250 µL stool samples spiked in with 20 µL ZymoBIOMICS™ Microbial Community Standard (Zymo Research). (n = 3) extracted on the Firefly NIMBUS 96, Qiagen BioSprint 96, and manually. (n = 3).

Mag-Bind® Plant DNA DS 96 Kit

The Mag-Bind® Plant DNA DS 96 Kit allows rapid and reliable isolation of high quality genomic DNA from a variety of plant species containing high amounts of polysaccharides and polyphenols. The system combines the robust lysing power of cationic detergent, cetyltrimethylammonium bromide (CTAB) with the rapid response of the Mag-Bind® particles to efficiently purify high quality DNA.

  • No organic extractions
  • Robust lysis method to handle diverse plant species
  • High yield of DNA suitable for most downstream applications

DNA Quality from Canola

Genomic DNA from canola leaf sample extracted manually and on Firefly NIMBUS 96 analyzed on Agilent’s TapeStation™ 2200.