Lanes 1-6: human whole blood, lanes 7-9 human saliva, lanes 10-12 dried human blood spot. M: 100 bp DNA marker. 15% of PCR products were loaded onto 1% agarose gel.
Amplification using bullfrog blood and tilapia blood extracted by E.Z.N.A.® Blood Direct PCR Kit as template, 10% PCR products were loaded onto 1.2% agarose gel. M: DL2000 DNA marker. C1: positive control, use bullfrog blood and tilapia blood as template. C2: negative controls, using double steamed water as template.