Omega Bio-tek’s E.Z.N.A.® M13 DNA Kits are designed to purify up to 10 µg of single-stranded DNA from up to 3 mL of phage supernatant. Yields of single-stranded DNA obtained using E.Z.N.A.® M13 DNA Kits are around 3-10 µg and reproducible when the isolations are performed from the same culture.
The E.Z.N.A.® M13 DNA Kit isolation procedures first call for the infected bacterial culture to be centrifuged to pellet the bacterial cells. MPG buffer is added to the supernatant to precipitate the phage particles. The samples are loaded on HiBind® M13 DNA Mini Columns or on to E-Z 96® DNA Plates. The specially designed HiBind® matrix will retain intact phage particles. These phage particles are lysed and bound to the HiBind® matrix after the addition of MPX Buffer. Contaminants such as protein are efficiently washed away with SPW Wash Buffer and pure ssDNA is eluted with Elution Buffer.