E-Z 96® Total RNA Kit II is designed to isolate total cellular RNA from all types of animal and human tissues. This kit allows simultaneous purification of 96 samples in less than 60 minutes. RNA purified using this kit is ready for applications such as RT-PCR*, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
The E-Z 96® Total RNA Kit II provides a fast and easy method for high throughput RNA isolation. This kit integrates efficient lysis of a phenol/guanidine buffer and the reversible binding properties of HiBind® matrix, a silica-based material, with the speed of the E-Z 96® plate format for fast processing of large sample numbers. Cells or tissues are homogenized with RNA-Solv® Reagent that inactivates RNases. Chloroform is added to separate the homogenate into aqueous and organic phases via centrifugation. The aqueous phase, which contains RNA, is adjusted with ethanol and then applied to the E-Z 96® RNA Plate to bind total RNA, while cellular debris and other contaminants are effectively washed away. High quality RNA is eluted in DEPC Water.