E-Z 96® Viral RNA Kit is designed for isolation of viral RNA from acellular fluids such as plasma, serum, urine, and cell culture supernatant. The procedure completely removes contaminants and enzyme inhibitors making viral RNA isolation fast, convenient, and reliable. This kit has been validated with hepatitis A and C, and HIV. The kit is also suitable for isolation of total RNA from cultured cells, tissues, and bacteria.
RNA purified using the E-Z 96® Viral RNA method is ready for applications such as RT-PCR*.
The E-Z 96® Viral RNA Kit uses the reversible binding properties of the silica-based HiBind® matrix combined with the speed of mini-column spin technology or vacuum manifold, to process multiple samples quickly and efficiently. The sample is lysed under denaturing conditions that inactivate RNases and protects the intact viral RNA from degradation. After adjusting the binding conditions, the samples are transferred to the E-Z 96® RNA Plate. With a brief centrifugation or vacuum step, the samples pass through the plate and the viral RNA binds to the HiBind® matrix. After 2 wash steps, purified viral RNA is eluted with RNase-free water.