The E.Z.N.A.® BAC/PAC DNA Kit is designed for rapid high-throughput purification of BACs, PACs, and P1s from small volume bacterial cultures. This kit is based on a modified alkaline lysis procedure that has been adapted for use with spin columns and high-throughput procedures. The protocol has been tested using a variety of low copy cosmids, BACs, PACs, P1s, and E.coli strains. In addition this kit can also be used for high copy plasmid isolation. Two protocols are provided in this handbook for your convenience. The new standard protocol provides a fast and reliable method for purification of BAC, PAC, P1, and plasmid DNA using a HiBind® DNA Mini Column. The second protocol is for the isolation of low copy number cosmids, BACs, PACs, and P1s.