The E.Z.N.A.® Bacterial RNA Kit is designed for isolation of high quality total RNA from variety of bacterial strains. Up to 1 x 109 log- phase bacterial cells can be processed. This kit uses an improved lysis procedure to ensure the complete lysis of bacterial cells. Purified RNA is suitable for downstream applications such as RT-PCR and hybridization techniques.
Total RNA purified using the E.Z.N.A.® Bacterial RNA Kit. RNA was extracted from lag phase E. coli cultures as follows: sample 1 from 0.5 mL culture, sample 2 from 1.0 mL culture, sample 3 from 2 mL culture, and sample 4 from 3 mL culture. Lanes 1-4 represent the first 100 µL elution. Total RNA (50% of total purified RNA) was analyzed on a denaturing formaldehyde agarose gel to demonstrate yield and quality of the RNA. M: CL5000 DNA Marker.