The E.Z.N.A.® Cycle-Pure Kit is designed for the rapid purification of single or double-stranded DNA from PCR or other enzymatic reactions. The purification procedure completely removes primers, nucleotides enzymes, salts, and other impurities from DNA sample. The DNA sample is simply mixed with buffer and spun through the HiBind DNA column. The DNA bound to the HiBind Matrix is washed and the clean concentrated DNA is eluted with deionized water or elution buffer. This convenient spin-column format eliminates the need for expensive resins or toxic organic compounds such as phenol and chloroform, thereby making it possible to process multiple samples in parallel. Purified DNA can be used in T-A ligations, sequencing, restriction enzyme digestion, and various other labeling reactions.
- Rapid – Purification of PCR products in 10 minutes
- Convenient – No Phenol/chloroform extractions
- Versatile – Spin and vacuum formats available
- High-quality – DNA is suitable for downstream applications