The E.Z.N.A.® FFPE DNA Kit is designed for fast and easy purification of DNA from formalin-fixed paraffin-embedded (FFPE) tissue sections. Paraffin removal can be performed using a xylene and ethanol method or an efficient heat treatment. Samples are incubated in a specialized lysis buffer along with Proteinase K to reverse crosslinking effectively releasing short and long DNA fragments. After adjusting the binding conditions with ethanol, the lysate is applied to the MicroElute® DNA Column to bind DNA. Cellular debris and proteins are effectively removed during the wash steps. High-quality purified DNA is then eluted in Elution Buffer or water and is ready for applications such as PCR and Next-Gen sequencing.

Product Number Preps Price
D3399-00 5 Preps $ 15.00
D3399-01 50 Preps $ 134.00
SKU: D3399 Category:

Product Description

D3399-2 D3399-1

Quality and yield of DNA with agarose gel analysis comparing Company A’s FFPE Kit and Omega Bio-tek’s E.Z.N.A.® FFPE DNA Kit. Analysis was completed on a 0.5% agarose gel. Lanes 1-3 represent Company A and lanes 4-6 represent Omega Bio-tek’s E.Z.N.A.® FFPE DNA Kit, M: 1 kb marker. FFPE DNA tissue starting material was ~2 mg post-deparaffinization. An overnight digestion was completed at 55°C. Samples were eluted in 60 µL elution buffer. O.D. ratio and yield calculated using a Thermo NanoDrop® 2000c.