The E.Z.N.A.® FFPE RNA Kit is specially designed for isolation of total RNA from formalin-fixed, paraffin-embedded tissue sections. This method can also be used for the preparation of total RNA from fibrous samples such as heart and muscles tissue. The sample is first processed in special buffer by heating to deactivate RNase’s and deparaffinization. Then the lysate is treated with Proteinase K to digest proteins. The cleared lysate is then applied to a HiBind RNA column to capture RNA. After two quick wash steps, purified RNA is eluted with water. While E.Z.N.A.® FFPE RNA Kit is optimized to minimize the effect of the formaldehyde modification, it is not recommended to use the RNA purified from this kit for downstream applications that requires full length RNA.
The result of electropherogram: T: add 200 µL ethanol, S: add 600 µL ethanol, M: CL5000 DNA marker. Conclusion: successful extraction of small fragments when the condition is added 600 µL ethanol. No matter the condition, is added 200 µL ethanol or 600 µL ethanol, a lot of large fragments can be extracted.