DNA ladder was run on a 2% agarose gel and 4 different fragment sizes (200 bp, 500 bp, 1 kb and 5 kb) were recovered using Omega Bio-tek’s Gel Extraction Kit and a Company Q kit for gel extraction following the manufacturer’s recommended protocols. DNA was analyzed on a 2% TBE agarose gel with the respective companies eluate being compared to the original amount used in the gel extraction procedure.
Recovery percentage of E.Z.N.A.® Gel Extraction Kit versus a comparable kit from Company Q. The input amounts were normalized to 100%. O.D. readings taken with Thermo’s NanoDrop 2000c.