E.Z.N.A.® Gel Extraction Kit

$10.00$225.00

Gel purification of DNA is a common technique used for the isolation of specific DNA fragments from reaction products. However, most methods either fail to completely remove agarose, causing problems in downstream manipulations or shear the DNA, resulting in very low yields. The E.Z.N.A.® Gel Extraction Kit uses HiBind® spin column technology to purify DNA bands 70 bp – 20 kb in length from all grades of agarose gels with up to 85% recovery. The DNA band of interest is excised from the gel, dissolved in binding buffer and applied to a HiBind® DNA spin column. Following three wash steps, DNA is eluted with deionized water or elution buffer and is ready for downstream applications such as ligations, PCR amplification, restriction enzyme digestion and various labeling reactions. This kit can also be used to purify DNA fragments from PCR products and enzymatic reactions. Two different spin column formats (V-spin and Q-spin) are available for this kit. V-spin columns have an attached cap, while the Q-spin columns are capless. The columns are otherwise identical in use and application. Either column can be used with either the vacuum or centrifugation protocols.

  • Rapid – DNA recovery from agarose gel in < 10 min
  • Reliable – Optimized buffers guarantee pure DNA
  • Safety – No organic extractions
  • High-quality – Purified DNA suitable for most applications
Product Number Preps Price
D2500-00 5 $10.00
D2500-01 50 $60.00
D2500-02 200 $225.00

 

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SKU: D2500 Categories: ,

Product Description

d2500-2

DNA ladder was run on a 2% agarose gel and 4 different fragment sizes (200 bp, 500 bp, 1 kb and 5 kb) were recovered using Omega Bio-tek’s Gel Extraction Kit and a Company Q kit for gel extraction following the manufacturer’s recommended protocols. DNA was analyzed on a 2% TBE agarose gel with the respective companies eluate being compared to the original amount used in the gel extraction procedure.

d2500-1

Recovery percentage of E.Z.N.A.® Gel Extraction Kit versus a comparable kit from Company Q. The input amounts were normalized to 100%. O.D. readings taken with Thermo’s NanoDrop 2000c.