$10.00$225.00

Product Number Preps Price
D2500-00 5 $10.00
D2500-01 50 $60.00
D2500-02 200 $225.00
Clear

Description

Gel purification of DNA is a common technique used for the isolation of specific DNA fragments from reaction products. However, most methods either fail to completely remove agarose, which can lead to problems in downstream manipulations; shear the DNA; or result in very low yields. The E.Z.N.A.® Gel Extraction kit uses HiBind DNA spin-column technology to purify DNA bands 70 bp – 20 kb in length from all grades of agarose gels with up to 85% recovery. The DNA band of interest is excised from the gel, dissolved in binding buffer, and applied to a DNA HiBind spin column. Following three wash steps, DNA is eluted with deionized water or elution buffer and is ready for downstream applications such as ligations, PCR amplification, restriction enzyme digestion, and various labeling reactions. This kit can be also used to purify DNA fragments from PCR products and enzymatic reactions. Two different spin column formats (V-Spin column and Q-Spin Column) are available for this kit.

  • Rapid – DNA recovery from agarose gel in 15 min
  • Reliable – Optimized buffers guarantee pure DNA
  • High-quality – Purified DNA suitable for most applications

DNA ladder was run on a 2% agarose gel and 4 different fragment sizes (200 bp, 500 bp, 1 kb, and 5 kb) were recovered using Omega Bio-tek’s Gel Extraction Kit and a Company Q kit for gel extraction following manufacturer’s recommended protocols. DNA was analyzed on a 2% TBE agarose gel with the respective companies eluate being compared to the original amount used in the gel extraction procedure.

Recovery percentage of E.Z.N.A.® Gel Extraction Kit versus a comparable kit from Company Q. The input amounts were normalized to 100%. O.D. readings taken with Thermo Scientific’s NanoDrop® 2000c.

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