The E.Z.N.A.® Micro RNA Kit combines the reversible binding properties of HiBind® matrix, a new silica-based material, with the highly efficient lysis ability of RNA-Solv® to extract micro and large (>200 nt) RNA from a wide variety of starting materials. Micro and large RNA are separated to allow for quantification into downstream applications.
The system uses RNA-Solv® to efficiently lyse cells and protect RNA from degradation. Following a chloroform separation step, RNA is application to a HiBind® RNA column where large RNA binds. The flow through is retained and applied to a HiBind® Micro RNA column to bind small RNA.