$21.00$227.00

Product Number Preps Price
R6875-00 5 $21.00
R6875-01 50 $227.00
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SKU: R6875 Category:

Description

The E.Z.N.A.® Mollusc RNA Kit is designed for the efficient isolation of total RNA from mollusc, arthropod, roundworm, flatworm, and other invertebrate tissue samples rich in mucopolysaccharides. Samples are homogenized and lysed in a high salt buffer containing CTAB, and extracted with chloroform to remove mucopolysaccharides and to denature proteins. Following a rapid precipitation step, binding conditions are adjusted and RNA is further purified using HiBind RNA spin columns. Purified RNA is suitable for downstream applications such as RT-PCR, Poly A+ (mRNA) purification, and hybridization techniques.


Total RNA purified using the E.Z.N.A.® Mollusc RNA Kit. Purified total RNA from various samples was isolated with the E.Z.N.A.® Mollusc RNA Kit. RNA was extracted from samples as follows: sample 1 from fire ants, sample 2 from earthworms, sample 3 from snails, sample 4 from abalone, sample 5 from slug, and sample 6 from winkles. Total RNA (10% of total purified RNA) was analyzed on a 1% agarose gel to demonstrate yield and quality of the RNA.

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