The E.Z.N.A.® Soil RNA Midi Kit is designed to isolate high quality total RNA from soil samples typically containing humic acid and inhibitors of RT-PCR. This kit uses a novel and proprietary method to isolate total RNA from a variety of environmental samples. Soil samples are homogenized and extracted with phenol/chloroform. A specially formulated suspension buffer is added to remove coloration and inhibitors. By using an innovative DNA Clearance Column, DNA is effectively removed without the need for DNase digestion. The filtrate containing the RNA is then adjusted with ethanol and applied to the HiBind® RNA column to which total RNA binds, while cellular debris and other contaminants are effectively washed away. High quality RNA is finally eluted in DEPC-treated sterile water. The entire procedure can be completed in under 1 hour.