$23.00$607.00

Catalog No Preps Price
D5542-00 5 $23.00
D5542-01 50 $160.00
D5542-02 200 $607.00

*This product will be discontinued on January 1, 2018 or when current stock levels are depleted.

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SKU: D5542 Category: Tags: ,

Description

E.Z.N.A.® SP Fungal Mini Kits are specially designed for rapid and reliable isolation of high-quality total cellular DNA from fungal species that contain high levels of phenolic compounds and polysaccharides. Up to 100 mg wet tissue (or 25 mg dry tissue) can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of Omega Bio-tek’s HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from fungal tissue lysates. The newly introduced homogenization columns provide a fast and easy tool for sample homogenization. Purified DNA is suitable for PCR, restriction digestion, and hybridization applications. There are no organic extractions, thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel.


Total cellular DNA purified using the E.Z.N.A.® SP Fungal DNA Kit. Purified total cellular DNA from various fungi was isolated with the E.Z.N.A.® SP Fungal DNA Kit. DNA was extracted from 50 mg fresh fungal tissue as follows: lane 1 from Ganoderma lucidum, lane 2 from Cordyceps sinesis, lane 3 from Agaricus campestris, and lane 4 from Russula foetens. Genomic DNA (5% of total purified DNA) was analyzed on a 0.8% agarose gel to demonstrate yield and quality of the DNA. M: Lambda Hind III DNA Marker.

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