The E.Z.N.A.® SQ Total RNA Kit is designed for isolating total RNA from animal, tissue and cultured cells. This solution based system can be easily scaled up and down based on the starting material. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifugation that are eliminated. RNA purified using the E.Z.N.A.® SQ Total RNA method can be directly used for applications such as RT-PCR*, Northern blotting, and other enzymatic reactions.
Yield of total RNA purified using the E.Z.N.A.® SQ Total RNA Kit. Purified total RNA from 50 mg rice leaf was isolated with the E.Z.N.A.® SQ Total RNA Kit. RNA was eluted in 30 µL DEPC-treated water and quantitated using Thermo Fisher’s NanoDrop® 2000.
Total RNA purified using the E.Z.N.A.® SQ Total RNA Kit. Purified total RNA from 50 mg plant leaf samples was isolated with the E.Z.N.A.® SQ Total RNA Kit. RNA was extracted from plant samples as follows: sample 1 from rice leaf, sample 2 from corn leaf, sample 3 from soybean leaf, sample 4 from tomato leaf, sample 5 from corn seeds, and sample 6 from soybean seeds. Total RNA (5% of total purified RNA) was analyzed on a 1% agarose gel to demonstrate yield and quality of the RNA.