DNA was isolated from 10 mg rat kidney using Omega Bio-tek’s Tissue DNA Kit. A serial dilution of recovered DNA was then used in SYBR Green real-time PCR targeting a 100 bp fragment of the GAPDH gene. Each reaction was performed in triplicate. The lines represent input DNA amounts of 10, 2, 0.4, 0.08, 0.0016 ng of DNA.
Purified genomic DNA from 10 mg rat kidney tissue was isolated with Company T, Company A, Company P, Omega Bio-tek’s Tissue DNA Kit and Company Q’s genomic DNA extraction kits following the manufacturer’s recommended protocols. 3% of eluted DNA was analyzed on a 0.8% agarose gel.