Total RNA purified using the E.Z.N.A.® Total RNA Kit II. Purified total RNA from mouse tissue samples was isolated with the E.Z.N.A.® Total RNA Kit II. Total RNA (300 ng/lane) was analyzed on a 1% agarose gel to demonstrate yield and quality of the RNA. M: CL5000 DNA Marker.
RNA was isolated from 10 mg chicken liver using the E.Z.N.A.® Total RNA Kit II. A serial dilution of recovered RNA was then used in a SYBR Green real-time RT-PCR targeting a 150 bp fragment of the &beta-actin. Each reaction was performed in 40 replicates. The lines represent input RNA amounts of 50, 5, 0.5, 0.05 and 0.005 ng (left to right) per reaction.