The E.Z.N.A.® Yeast RNA Kit allows convenient isolation of high-quality total RNA from a wide variety of yeast species. Up to 2 x 107 log-phase cultured yeast cells can be processed. The system combines the reversible nucleic acid-binding properties of HiBind® matrix with the speed and versatility of spin column technology to yield approximately 30 µg of RNA, with an A260/A280 ratio of 1.7-1.9. Purified RNA is suitable for downstream applications such as RT-PCR, DD-PCR, and hybridization techniques. There are no organic extractions, thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel.