E.Z.N.A.® Circulating DNA Kit
The E.Z.N.A.® Circulating DNA Kit provides a rapid and easy method for the isolation of circulating DNA from plasma, serum, and other acellular body fluids. The kit is designed to isolate cell-free DNA from various amounts of fresh or frozen plasma/serum samples ranging from 1 mL up to 4 mL and allows flexible elution volumes ranging from 30 µL to 150 µL. DNA purified using the E.Z.N.A.® Circulating DNA method is ready for applications such as PCR, circulating detection, and next-generation sequencing.
E.Z.N.A.® Circulating DNA Kit uses a silica spin column-based purification strategy combining the reversible nucleic acid-binding properties of our HiBind® matrix with the speed of mini column centrifugation. The kit also uses funnel adaptors to allow large volumes of liquid to be applied to the mini columns using a vacuum manifold for eluting concentrated circulating DNA. A specially formulated buffer system allows circulating DNA to bind to the HiBind® matrix. Samples are lysed under denaturing conditions and then transferred to the E.Z.N.A.® Circulating Column to bind DNA and cellular debris, hemoglobin, and other proteins are washed away.