E.Z.N.A.® Circulating DNA Kit

$205.00$718.00

The E.Z.N.A.® Circulating DNA Kit provides a rapid and easy method for the isolation of circulating DNA from plasma, serum, and other acellular body fluids. The kit is designed to isolate cell-free DNA from various amounts of fresh or frozen plasma/serum samples ranging from 1 mL up to 4 mL and allows flexible elution volumes ranging from 30 µL to 150 µL. DNA purified using the E.Z.N.A.® Circulating DNA method is ready for applications such as PCR, circulating detection, and next-generation sequencing.

E.Z.N.A.® Circulating DNA Kit uses a silica spin column-based purification strategy combining the reversible nucleic acid-binding properties of our HiBind® matrix with the speed of mini column centrifugation. The kit also uses funnel adaptors to allow large volumes of liquid to be applied to the mini columns using a vacuum manifold for eluting concentrated circulating DNA. A specially formulated buffer system allows circulating DNA to bind to the HiBind® matrix. Samples are lysed under denaturing conditions and then transferred to the E.Z.N.A.® Circulating Column to bind DNA and cellular debris, hemoglobin, and other proteins are washed away.

Catalog No. Preps Price
D3091-00 5 preps $205.00
D3091-01 50 preps $718.00
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SKU: D3091 Category:

Product Description

Electropherogram overlay of purified DNA from 4 mL serum. 4 mL of unspiked serum was purified using kits from Omega Bio-tek and Company Q following manufacturer’s recommended protocols. Purified DNA was analyzed on Agilent’s TapeStation® 2200.

Additional Information

Size

5 preps, 50 preps