E.Z.N.A.® FastFilter Plasmid Midi Kit offers a novel and reliable method of isolating up to 200 µg of plasmid DNA in less than 40 minutes. A lysate clearance syringe replaces a time consuming centrifugation and transfer step, saving 20-30 minutes. This kit combines HiBind® membrane technology with the time-tested consistency of alkaline SDS lysis of bacterial cells to deliver high-quality plasmid DNA. Plasmid purification using the E.Z.N.A.® Fastfilter Plasmid Midi Kit follows a simple bind-wash-elute procedure thereby making it possible for multiple samples to be processed. Although yields vary according to plasmid copy number, E.coli strain, and conditions of growth, 50 mL of an overnight culture in LB medium will typically produce 200 µg of high-copy number plasmid DNA. When working with low-copy number plasmids, one can increase the starting culture volume up to 100 mL. High-quality DNA is ready for immediate use in routine molecular biology applications such as automated fluorescent sequencing, restriction enzyme digestion, and transfection screening.
- Rapid – Purification of plasmid DNA in < 40 min
- Safe – No phenol/chloroform extractions
- Versatile – Spin and vacuum formats available
- High-quality – DNA is suitable for most downstream applications