Gel purification of DNA is a common technique for the isolation of specific fragments from reaction mixtures. However, most methods either fail to completely remove agarose or shear DNA which can lead to problems in downstream manipulations. The E.Z.N.A.® Gel Extraction Kit uses proprietary chemistry and HiBind® technology to recover DNA fragments between 70 bp and 20 kb with yields exceeding 85%. The DNA band of interest is excised from the gel, dissolved in Binding Buffer, and transferred to a HiBind® DNA Mini Column. Following three rapid wash steps, DNA is eluted with the Elution Buffer and is ready for other applications. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.