$74.00

Product Number Preps Price
D7043-01 50 $74.00
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SKU: D7043 Category:

Description

The E.Z.N.A.® HP Plasmid Mini Kit I is designed to isolate up to 30 µg of high-quality plasmid DNA from 1-5 mL bacterial cultures from strains that are endonuclease positive. The HP Plasmid DNA Kit includes a protease digestion step to remove endonucleases and proteins from the sample.  Plasmid DNA purification is simplified with HiBind® Mini Column technology into three quick steps: bind, wash, and elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, and manual/automated fluorescent DNA sequencing.

  • Rapid – Purification of plasmid DNA in 30 minutes or less
  • Safe – No phenol/chloroform extractions
  • Versatile – Spin and vacuum formats available
  • High-quality – DNA is suitable for downstream applications

Comparison of DNA purified using the E.Z.N.A.® HP Plasmid Mini Kit I and the standard E.Z.N.A.® Plasmid Mini Kit (D6943). Plasmid DNA was isolated from 2 mL HB101 and DH5α bacterial cultures grown overnight in LB culture using the E.Z.N.A.® HP Plasmid Mini Kit I and the standard E.Z.N.A.® Plasmid Mini Kit I. DNA was eluted in 100 µL elution buffer and 3% of total purified DNA was analyzed on a 0.8% agarose gel. Lanes H1: HB101/HP Plasmid Mini Kit, S1: HB101/standard plasmid mini kit.

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