E.Z.N.A.® HP Total RNA Kit

$21.00$644.00

The E.Z.N.A.® HP Total RNA Isolation Kit provides a rapid and easy method for RNA isolation from a small amount of cultured eukaryotic cells or tissue. This kit allows single or simultaneous processing of multiple samples in less than 40 minutes. Normally, 1 x 107 eukaryotic cells or 25-30 mg tissue can be used in a single experiment. There is no need for phenol/chloroform extractions, time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl. RNA purified using the E.Z.N.A.® HP Total RNA method is ready for applications such as RT-PCR, RT-qPCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

  • Rapid – 40 minutes or less
  • Safe – No phenol/chloroform extractions
  • Versatile – Spin and vacuum formats available
  • Quality – Purified DNA suitable for a variety of downstream applications

View application notes:

Product Number Preps Price
R6812-00 5 $21.00
R6812-01 50 $195.00
R6812-02 200 $644.00

 

 

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SKU: R6812 Category:

Product Description

r6812-1

Total RNA isolated from various amounts of liver tissue using Omega Bio-tek’s E.Z.N.A.® HP Total RNA Kit and Company Q’s Total RNA Kit with DNA removal columns following the standard protocol for each respective company. Yield was calculated using ThermoFisher’s NanoDrop 2000. On average, Omega Bio-tek’s HP Total RNA Kit had 9.79% higher yield than Company Q.

r6812-2

Total RNA isolated from various amounts of liver tissue using Omega Bio-tek’s E.Z.N.A.® HP Total RNA Kit and Company Q’s Total RNA Kit with DNA removal columns following the standard protocol for each respective company. 1/10th of the eluate was analyzed on an agarose gel. Lanes 1-4 represent Company Q and lanes 5-8 represent Omega Bio-tek’s HP Total RNA Kit from sample sizes at 40, 20, 10 and 5 mg, respectively. Lane 9 is 1 kb ladder.