The E.Z.N.A.® HP Total RNA Isolation Kit provides a rapid and easy method for RNA isolation from a small amount of cultured eukaryotic cells or tissues. This kit allows single or simultaneous processing of multiple samples in less than 40 minutes. Normally, 1 x 107 eukaryotic cells or 25-30 mg tissue can be used in a single experiment. There is no need for phenol/chloroform extractions, time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl. RNA purified using the E.Z.N.A.® HP Total RNA method is ready for applications such as RT-PCR, RT-qPCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Total RNA isolated from various amounts of liver tissue using Omega Bio-tek’s E.Z.N.A.® HP Total RNA Kit and Company Q’s Total RNA Kit with DNA removal columns following the standard protocol for each respective company. 1/10th of the eluate was analyzed on an agarose gel. Lanes 1-4 represent Company Q and lanes 5-8 represent Omega Bio-tek’s HP Total RNA Kit from sample sizes as 40, 20, 10, and 5 mg, respectively. Lane 9 is 1 kb ladder.