$21.00$644.00

Product Number Preps Price
R6812-00 5 $21.00
R6812-01 50 $195.00
R6812-02 200 $644.00

 

 

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SKU: R6812 Category:

Description

The E.Z.N.A.® HP Total RNA Isolation Kit provides a rapid and easy method for RNA isolation from a small amount of cultured eukaryotic cells or tissues. This kit allows single or simultaneous processing of multiple samples in less than 40 minutes. Normally, 1 x 107 eukaryotic cells or 25-30 mg tissue can be used in a single experiment. There is no need for phenol/chloroform extractions, time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl. RNA purified using the E.Z.N.A.® HP Total RNA method is ready for applications such as RT-PCR, RT-qPCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.


Total RNA isolated from various amounts of liver tissue using Omega Bio-tek’s E.Z.N.A.® HP Total RNA Kit and Company Q’s Total RNA Kit with DNA removal columns following the standard protocol for each respective company. Yield was calculated using Thermo Fisher’s NanoDrop® 2000. On average, Omega Bio-tek’s HP Total RNA Kit had 9.79% higher yields than Company Q.

Total RNA isolated from various amounts of liver tissue using Omega Bio-tek’s E.Z.N.A.® HP Total RNA Kit and Company Q’s Total RNA Kit with DNA removal columns following the standard protocol for each respective company. 1/10th of the eluate was analyzed on an agarose gel. Lanes 1-4 represent Company Q and lanes 5-8 represent Omega Bio-tek’s HP Total RNA Kit from sample sizes as 40, 20, 10, and 5 mg, respectively. Lane 9 is 1 kb ladder.

 

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