Electropherogram overlay of purified DNA from 4 mL serum. 4 mL of unspiked serum was purified using kits from Omega Bio-tek and Company Q following manufacturer’s recommended protocols. Purified DNA was analyzed on Agilent’s TapeStation 2200.
Electropherogram overlay of purified DNA from 1 mL serum. 100 ng of 200 bp sheared bacterial genomic DNA was spiked into 1 mL of serum and extracted using Omega Bio-tek’s Mag-Bind® cfDNA Kit and a comparable column-based kit from Company Q following manufacturer’s recommended protols. Purified DNA was analzyed on Agilent’s TapeStation® 2200. The Omega Bio-tek kit was able to capture the circulating, cell-free DNA with no genomic DNA contamination. In contrast, Company Q’s eluate contained high molecular weight fragments indicating the presence of genomic DNA in the circulating DNA isolation.
qPCR comparison for 200 bp fragments. Real-time PCR with human specific primers was performed on triplicates of undiluted and 10-fold dilutions of DNA isolated using Omega Bio-tek’s Mag-Bind® cfDNA Kit and a comparable column-based kit from Company Q.