Many high-throughput applications, such as sequencing and genotyping, require the input DNA concentration to be within a certain range for optimal results. Traditionally, a tedious process of quantification, calculation and concentration adjustment must be carried out to normalize the DNA samples.
The Mag-BIND® EquiPure Normalizer Kit completely eliminates the need to quantify and aliquot DNA, saving time, quantification bias and tip cost. Using our proprietary Mag-BIND® Normalizer Beads and binding buffer system, input DNA of various quantities is simply bound, washed and eluted to a final normalized product. The magnetic beads have a limited binding capacity and therefore allow a predefined amount of DNA to be captured and eluted.
The Mag-BIND® EquiPure Normalizer Kit is fully automatable on multiple liquid handling platforms including Hamilton STAR, Tecan Evo, Caliper Sciclone, and Beckman Coulter Biomek instruments.
The Mag-BIND® EquiPure Normalizer Kit allows user to adjust the quantity of DNA that binds to the magnetic beads and elution volumes can be adjusted to vary DNA concentrations to users needs.
- DNA output concentrations +/- 10%
- No quantification required
- Normalizes genomic DNA or NGS libraries
Varying amounts of 16S NGS libraries were prepared and normalized with the Mag-BIND® EquiPure Normalization Kit. DNA yield was quantified using Promega’s QuantiFluor® dsDNA system.
Varying amounts of Illumina TruSeq Stranded mRNA libraries were prepared and normalized with the Mag-BIND® EquiPure Normalization Kit. DNA yield was quantified using Promega’s QuantiFluor® dsDNA system.