DNA was isolated from FFPE samples using the Mag-Bind® FFPE DNA Kit and Company A’s FFPE DNA and RNA isolation kit following standard protocols. A serial dilution of recovered DNA was then used in a SYBR® real-time qPCR. Each reaction was performed in triplicate. The points represent input DNA amounts of 50, 5, 0.5 and 0.05 ng (right to left) per reaction.
Comparison of Company A’s silica spin column method vs. Omega Bio-tek’s Mag-Bind® FFPE DNA Kit.