Mag-Bind® FFPE RNA Kit
The Mag-Bind® FFPE RNA kit is specially designed for isolation of total RNA from formalin-fixed, paraffin-embedded tissue sections. The sample is first processed in special buffer by heating to deactivated RNases/DNase and deparaffinization. Then the lysate is treated with Proteinase K to digest proteins. The cleared lysate is then mixed with Mag-Bind particles to capture the capture RNA. After few quick wash steps, purified RNA can be eluted with water or low salt buffer. The standard protocol uses a heating step instead of xylene to remove paraffin from FFPE sample. The alternative protocol uses traditional xylene extraction to remove paraffin. After the paraffin removal step, samples are lysed in FTL Buffer with digestion of Proteinase K. The lysate is then heated to denature the proteinase and mixed with MSL Buffer and magnetic particles to bind the nucleic acid on the surface of Mag-Bind particles. After two quick wash steps, purified DNA is eluted with DNA Elution Buffer or nuclease-free water.