30 mg canola seeds were extracted in triplicate according to the manufacturer’s recommended protocol and eluted in 100 µL. DNA was analyzed on NanoDrop 2000x.
30 mg canola seeds were ground using a Spex GenoGrinder 2010 and 4 mm stainless steel beads. Each extraction was performed in triplicate according to the manufacturer’s recommended protocol and eluted in 100 µL. A 20 µL SYBR Green-based real time PCR was performed in triplicate on a ~200 bp amplicon. 4 µL DNA template was used in each reaction.
Amplification of DNA isolated from rice seeds using the Mag-Bind® Plant DNA Plus Kit. qPCR was performed in triplicate. Samples were diluted 10X and difference between the diluted and undiluted samples were 3.18 cycles, indicating DNA is free of inhibition.