$205.00$9,225.00

Product Number Preps Price
M1300-05 5 mL $205.00
M1300-08 50 mL $1,128.00
M1300-50 500 mL $9,225.00
Clear

Description

Omega Bio-tek’s Mag-Bind® SeqDTR is designed for efficient and reliable removal of unincorporated dye terminators from sequencing reactions. The system combines Omega Bio-tek’s proprietary chemistry with the reversible nucleic acid-binding properties of paramagnetic beads to eliminate excess nucleotides, primers, salts, and unincorporated dye terminators. This kit is designed for both manual and fully automated purification of sequencing products, and the protocol has been successfully automated on liquid handlers such as Beckman Coulter’s Biomek® FX/NX, and the Hamilton Microlab® STAR/STARlet. With the high recovery rate from the Mag-Bind® SeqDTR Kit, typically only 1/16 or 1/32 of the ABI Big Dye reagents are needed for sufficient signal strength when compared to sephadex-based clean ups.

  • Read lengths averaging over 800 bps (Min Phred 20)
  • Adjustable to automated liquid handlers
  • Significant cost savings compared to sephadex-based clean ups
  • Available in 96- or 384-well formats

Comparison of magnetic bead-based big dye clean up chemistries. Purified 1.8 kb PCR fragments were sequenced from each company using the manufacturer’s recommended protocols. The median of 16 samples per company are used in the representations above. A 5 µL sequencing reaction was performed using a 1/32 dilution of Applied Biosystems BigDye Terminator v3.1 chemistry. DNA was analyzed on an Applied Biosystems 3730XL.

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