Mag-Bind® TotalPure NGS

$100.00$3,400.00

Mag-Bind® TotalPure NGS offers an easy-to-use, reliable solution for the purification of both DNA and RNA for next-generation sequencing workflows with high recovery rates. Mag-Bind® TotalPure NGS is capable of selectively binding fragments depending on the reagent to sample ratio used, giving the user flexibility to perform left, right or double-sided size selection. This product is designed for both manual and fully automated purification of DNA and RNA samples, as well as for the purification of PCR products. The system combines Omega Bio-tek’s proprietary chemistries with reversible nucleic acid-binding properties of magnetic beads to selectively bind fragments larger than 100 bp and eliminate excess nucleotides, primers, and small, non-targeted products such as primer-dimers. Purified DNA and RNA is suitable for a variety of downstream applications such as NGS library preparation, microarrays, automated fluorescent sequencing and restriction enzyme digestion.

Product No. Preps Price
M1378-00 5 mL $100
M1378-01 50 mL $550
M1378-02 500 mL $3400
Clear
SKU: M1378 Categories: , ,

Product Description

m1378-1

Electropherogram overlay of the double-sided size selection on sheared gDNA at 0.8x/0.7x ratio set using Omega Bio-tek’s Mag-Bind® TotalPure NGS and a comparable kit from Company A following manufacturer’s recommended protocols. The DNA was eluted in 25 µL and analyzed on Agilent’s TapeStation 2200.


m1378-3

10 µL of RNA at 50 ng/µL and 5 ng/µL was cleaned up with Omega Bio-tek’s Mag-Bind® TotalPure NGS following manufacturer’s recommended protocols. The RNA was eluted in 20 µL and analyzed on Agilent’s TapeStation 2200. Recovery rates were 85-92% respectively.

m1378-2

10 µL of 50 bp ladder was purified with Omega Bio-tek’s Mag-Bind® TotalPure NGS and a comparable product from Company A following manufacturer’s recommended protocols. The DNA was eluted in 20 µL and analyzed on Agilent’s TapeStation 2200.

m1378-4m1378-5

Next-generation sequencing libraries prepared from 350 ng sheared genomic DNA using Kapa Biosystem’s HyperPrep Kits (KK8504) and Omega Bio-tek’s Mag-Bind® TotalPure NGS on the Hamilton Microlab STAR. Mag-Bind® TotalPure NGS was used for 2 clean up steps (0.8x and 1.0x) following Kapa Biosystems recommended protocol for clean-up. DNA was analyzed on Agilent’s TapeStation 2200 following library construction.

Additional Information

Size

5 mL, 50 mL, 500 mL