The Mag-Bind® Total RNA Kit provides a novel technology for total RNA isolation. This kit allows the rapid and reliable isolation of high-quality total cellular RNA from a wide variety of cells and tissues. The system combines the efficient nucleic acid-binding properties of Mag-Bind® Particles with the E.Z.N.A.® RNA isolation system to provide superior quality RNA. Unlike column-based systems, the binding of nucleic acids to magnetic particles occurs in solution resulting in increased binding kinetics and binding efficiency. Particles are also completely re-suspended during the wash steps of the purification protocol, which improves removal of contaminants and increases nucleic acid purity. Mag-Bind® Total RNA procedure can be fully automated with most robotic workstations.
Mag-Bind® Total RNA Kit vs. Company Q’s silica spin column-based method. RNA was isolated according to manufacturer’s recommended protocols. RNA quality was assessed via Agilent’s TapeStation® 2200 and RNA quality determined via Thermo NanoDrop® 2000c. Omega Bio-tek’s Mag-Bind® Total RNA Kit had higher yields when comparing the amount of tissue input per extraction.