Plasmid DNA was isolated according to manufacturer’s recommended protocols from 0.8 mL LB cultures grown for 24 hours. Each protocol was performed in triplicate with the average data shown above. DNA was quantified with Thermo Scientific NanoDrop 2000c.
Endotoxins in plasmid DNA preps. Plasmid DNA was isolated from 0.8 mL LB cultures following each manufacturer’s recommended protocol. Endotoxin levels were determined with Thermo Scientific’s Pierce LAL Chromogenic Endotoxin Quantitation Kit.