Environmental metagenomics involves dealing with a nearly infinite number of different combinations of microbiome communities and sample types. Current commercial extraction kits are designed for speed and ease-of-use, and are not nearly robust enough to consistently extraction an adequate yield and representation of DNA from the resident microbiome for downstream analyses.
Omega Bio-tek has partnered with the Extreme Microbiome Project (XMP) to develop the Mag-Bind® Universal Metagenomics Kit for DNA extraction from organisms found in extreme environments that is also compatible for most environmental sample types. This new kit employs a hybrid, self-customizable protocol that covers proper pre-treatment for a variety of sample types, a core multi-lysis (mechanical, enzymatic, and chemical) extraction protocol, along with other sample type-specific steps. This approach provides investigators the ability to use one single kit to optimize and maintain their DNA extraction method for consistent results and analysis.
Omega Bio-tek, Inc. would like to thank the Extreme Microbiome Project (XMP) for their collaboration in co-developing this product.
Average yields from Paleomats (ancient microbial mats associated with former lake beds) — TF3B1 and VV8a — were collected from Antarctica and DNA was extracted using kits from Omega Bio-tek and other manufacturers, following recommended protocols.
Average yield from Rhizosphere samples. Harvested microorganisms were pelleted and resuspended in 1 mL of PBST. 100 µL of this resuspension was spiked with 3 µL of ZymoBIOMICS™ Microbial Community DNA Standard and DNA was extracted using kits from Omega Bio-tek and Company Q, following manufacturer’s recommended protocols. DNA was quantified with Thermo Scientific NanoDrop® 2000c.