Average yields from Paleomats (ancient microbial mats associated with former lake beds) — TF3B1 and VV8a — were collected from Antarctica and DNA was extracted using kits from Omega Bio-tek and other manufacturers following recommended protocols.
Average yield from Rhizosphere samples. Harvested microorganisms were pelleted and resuspended in 1 mL of PSBT. 100 µL of this resuspension was spiked with 3 µL of ZymoBIOMICS™ Microbial Community DNA Standard and DNA was extracted using kits from Omega Bio-tek and Company Q following manufacturer’s recommended protocols. DNA was quantified with Thermo Scientific NanoDrop® 2000c.