Mag-BIND® Viral DNA/RNA Kit is designed for the rapid and reliable isolation of viral RNA and viral DNA from whole blood, serum, plasma, saliva and other body fluids. The Mag-BIND® magnetic beads technology enables purification of high-quality nucleic acids that are free of proteins, nucleases, and other impurities. In addition to easily being adapted with automated systems, this procedure can also be scaled up or down depending on the amount of starting sample. The purified nucleic acids are ready for direct use in downstream applications such as amplification or other enzymatic reactions.
Nucleic acid was isolated from 200 µL of human whole blood with Omega Bio-tek’s Mag-BIND® Viral DNA/RNA Kit and a comparable kit from Company A using the manufacturer’s recommended protocols. The extractions were eluted in 100 µL. Three concentrations of template were used as templates in a SYBR® Green-labeled qPCR reaction. Each reaction was performed in quadruplicate and the mean Ct value is depicted in the above figure.