HBV virus (in quantities of 10 and 1 infectious unit[s]) was spiked into 200 µL of human serum. Viral nucleic acid was isolated with Omega Bio-tek’s Mag-Bind® Viral DNA/RNA Kit and with a comparable kit from Company A according to recommended protocols. 5 µL of template was used for a SYBR Green-labled qPCR reaction which was replicated 4 times. The resulting mean Ct values are shown in the above figure.
Nucleic acid was isolated from 200 µL of human whole blood with Omega Bio-tek’s Mag-Bind® Kit and a comparable kit from Company A using the manufacturer’s recommended protocols. The extractions were eluted in 100 µL. Three concentrations of template were used as templates in a SYBR Green-labeled qPCR reaction. Each reaction was performed in quadruplicateand the mean Ct value is depicted in the above figure.