The E.Z.N.A.® Mollusc DNA Kit is designed for efficient purification of genomic DNA from molluscs, arthropods, round worms, flatworms and other invertebrate tissues rich in mucopolysaccharides. Fresh and frozen samples that have been preserved in alcohol or DNE can be used with this kit. This procedure relies on the well established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the selective DNA binding of HiBind matrix. Samples are homogenized and chloroform is added to remove mucopolysaccharides. Following a rapid alcohol precipitation step, DNA is further purified and all salts, proteins and other contaminants are removed. High-quality genomic DNA is suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization techniques.
- Rapid – DNA isolation under 20 minutes following lysis
- Reliable – Optimized buffer system guarantee pure DNA every time
- High-quality – Purified DNA suitable for any application
- Flexible – Optimized buffer system can isolate DNA from a wide range of samples
Genomic DNA purified using the E.Z.N.A.® Mollusc DNA Kit. Purified genomic DNA from various mollusc tissue was isolated with the E.Z.N.A.® Mollusc DNA Kit. DNA was extracted from 30 mg mollusc tissue as follows: sample 1 from oyster sticking muscle, sample 2 from oyster muscle, sample 3 from mussel muscle, and sample 4 from river snail. Genomic DNA (10% of total purified DNA) was analyzed on a 1% agarose gel to demonstrate yield and quality of the DNA. M: CL5000 DNA Marker.
Amplification of genomic DNA isolated from 4 mollusc tissue samples using the E.Z.N.A.® Mollusc DNA Kit. DNA from mollusc tissue samples was isolated with the E.Z.N.A.® Mollusc DNA Kit as follows: sample 1 from oyster sticking muscle, sample 2 from oyster muscle, sample 3 from mussel muscle, and sample 4 from river snail. DNA was purified from 4 types of mollusc tissue samples. A total of 2 µL of each eluate was amplified with a PCR master mix and SSR primers to determine whether inhibitors were present in the eluted DNA. Each lane contains 20% PCR product separated on a 2% agarose gel. M: DL2000 DNA Marker. N: Negative control.