E.Z.N.A.® Plasmid Mini Kit I, Q(capless) Spin


The E.Z.N.A.® Plasmid Mini Kit I is designed to isolate up to 30 µg of high quality plasmid DNA from 1 – 5 mL of bacterial cultures in 30 minutes or less. This kit uses a modified alkaline lysis method to lyse the cells and separate genomic DNA from plasmid DNA. Plasmid DNA purification is further simplified by using our HiBind® mini column technology in three quick steps: bind, wash and elute. Purified plasmid DNA is ready for a wide variety of downstream applications including routine screening, restriction enzyme digestion, DNA sequencing, cloning, transformation and transfection. The E.Z.N.A.® Plasmid DNA Mini Kit I is available with two different types of columns — V-spin columns have an attached cap (D6943), while Q-spin columns are capless (D6942). The columns are otherwise identical in use and application. Either column can be used with both the vacuum or centrifugation protocol.

  • Rapid – Purification of plasmid DNA in 30 minutes or less
  • Safe – No Phenol/chloroform extractions
  • Versatile – Spin and vacuum formats available
  • Quality – DNA is suitable for various downstream applications

Product Number Preps Price
D6942-00 5 $13.00
D6942-01 50 $50.00
D6942-02 200 $170.00

SKU: D6942 Category: Tags: ,

Product Description


DNA concentration comparison. 4 mL DH5α cultures transformed with pGEM vector were performed according to manufacturer’s recommended protocols. Plasmid DNA concentration determined by optical density measurements with NanoDrop 2000c. Total elution volume used was 50 µL.