E.Z.N.A.® Plasmid Mini Kit I, V(capped) Spin
The E.Z.N.A.® Plasmid Mini Kit I is designed to isolate up to 30 µg of high quality plasmid DNA from 1 – 5 mL bacterial cultures in 30 minutes or less. This kit uses a modified alkaline lysis method to lyse the cells and separate genomic DNA from plasmid DNA. Plasmid DNA purification is further simplified by using our HiBind® mini column technology in three quick steps: bind, wash and elute. Purified plasmid DNA is ready for a wide variety of downstream applications including routine screening, restriction enzyme digestion, DNA sequencing, cloning, transformation and transfection. The E.Z.N.A.® Plasmid DNA Mini Kit I is available with two different types of columns — V-spin columns have an attached cap (D6943), while Q-spin columns are capless (D6942). The columns are otherwise identical in use and application. Either column can be used with both the vacuum or centrifugation protocol.
- Rapid – Purification of plasmid DNA in 30 minutes or less
- Safe – No phenol/chloroform extractions
- Versatile – Spin and vacuum formats available
- Quality – DNA is suitable for a variety of downstream applications