The E.Z.N.A.® Plasmid Mini Kit I is designed to isolate up to 30 µg of high-quality plasmid DNA from 1-5 mL bacterial cultures in 30 minutes or less. Plasmid DNA purification is simplified with HiBind® Mini Column technology into three quick steps: bind, wash, and elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, and manual/automated fluorescent DNA sequencing.
The E.Z.N.A.® Plasmid Mini Kit is available with 2 different types of columns: V-spin columns have an attached cap (D6943), while Q-spin columns are capless (D6942). The columns are otherwise identical in use and application. Either column can be used with both the vacuum or centrifugation protocols.
- Rapid – Purification of plasmid DNA in 30 minutes or less
- Safe – No phenol/chloroform extractions
- Versatile – Spin and vacuum formats available
- High-quality – DNA is suitable for downstream applications