RNA-Solv® Reagent is a one reagent system for the isolation of total RNA from cells and tissues. It is offered in 2 formats — concentrated and ready-to-use. Phenol is added by the user in the concentrated format whereas it is pre-added in the ready-to-use format. RNA-Solv® reagent is a single-phase solution of phenol and guanidine isothiocyanate and follows a modified single-step RNA isolation method developed by Chomczynski and Sacchi (1). The sample is homogenized and lysed in RNA-Solv® reagent, which maintains the integrity of the RNA while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.
This method is suitable for a wide range of starting material: up to 1 g of tissue or 1×108 cells of human, animal, plant or bacterial origin. The simplicity of the RNA-Solv® reagent method allows simultaneous processing of a large number of samples. The entire procedure can be complete in one hour. Total RNA prepared can be used for Northern blot analysis, dot blot hybridization, poly (A)+ selection, in vitro translation, RNase protection assay and molecular cloning. For use in amplification by thermal cycling, treatment of the isolated RNA with RNase-free DNase I is recommended when the two amplimers lie within a single exon.
(1) Chomczynski. P and Sacchi. N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. Apr;162(1):156-9.
|R6830-01IN (No phenol)||40 mL||$101.00|