The E.Z.N.A.® RNA Lock stabilizer reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, and white blood cells into the RNA Lock stabilizer reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at -20°C.
Total RNA purified after storing in the E.Z.N.A.® RNA Lock Stabilizer Reagent. Purified total RNA from 20 mg mouse liver was isolated with the E.Z.N.A.® Total RNA Kit II. The figures show biological samples stored in RNA Lock can last up to 1 day at 30-37°C, 6 days at room temperature (20-25°C), and 1 month at 4°C conditions. Total RNA (10% of total purified RNA) was analyzed on a 1% agarose gel to demonstrate yield and quality of the RNA.