DNA was isolated from 100 mg peanut leaf using Omega Bio-tek’s SP Plant DNA Kit. A serial dilution of recovered DNA was then used in SYBR Green real-time PCR targeting a 91 bp fragment of the Alah1 gene. Each reaction was performed in triplicate. The lines represent input DNA amounts of 30, 3, 0.3, and 0.03 ng of DNA.
Average yields obtained from 100 mg of fresh leaf tissue from various plant samples.