Expected yield from 200 µL whole blood. Genomic DNA was isolated from seven 200 µL whole blood samples stored in EDTA using the SQ Blood DNA Kit. O.D. ratio and yield was calculated using a Thermo NanoDrop® 2000c.
The E.Z.N.A.® SQ Blood DNA Kit is designed for isolating high molecular weight genomic DNA from fresh, frozen, and anti-coagulated whole blood. The method can also be used for preparation of genomic DNA from buffy coat, bone marrow or cultured cells. The kit allows single or multiple, simultaneous processing of samples in under 90 minutes. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifugation are eliminated. DNA purified using the E.Z.N.A.™ SQ Blood DNA method is ready for applications such as PCR*, Southern blotting, and restriction digestion.