Mag-Bind® FFPE DNA/RNA 96 Kit

Mag-Bind® FFPE DNA/RNA 96 Kit is designed for the sequential isolation of DNA and RNA in separate eluates from the same formalin-fixed, paraffin-embedded (FFPE) tissue sample. The purification of DNA and RNA from the same sample enables a more comprehensive analysis from a precious sample source. The protocol utilizes a specially formulated buffer system that not only partially reverses the formaldehyde-induced crosslinking but also ensures DNA and RNA are differentially purified with no cross-contamination. The kit can also be used for purification of only DNA or only RNA from FFPE samples if sequential
isolation is not desired. Magnetic bead-based extraction makes it suitable for both manual as well as automated processing. Purified DNA and RNA are suitable for a variety of downstream applications including SNP analysis, next generation sequencing, and genotyping.

The Mag-Bind® FFPE DNA/RNA 96 Kit integrates a unique buffer system with the highly efficient binding properties of Mag-Bind® technology to isolate total DNA and RNA in separate eluates from the same FFPE sample. The protocol utilizes non-toxic mineral oil in combination with heat for efficient deparaffinization of the FFPE sample eliminating the use of hazardous xylene.

Samples are first lysed in FDR Buffer aided by the presence of Proteinase K enzyme. The lysate is then heated to denature the proteinase and reverse the chemical crosslinking of the nucleic acids. Post-heating, the lysate is mixed with MB4 Buffer and Mag-Bind® Particles CH to bind DNA to the particles. The RNA-containing supernatant is saved and a second binding step is completed with addition of isopropanol to bind RNA to the Mag-Bind® Particles CH. This results in separation of DNA and RNA into two fractions. Mag-Bind® Particles bound to DNA and RNA are individually washed and nucleic acids are eluted in two different tubes for further analyses. Only DNA or RNA can be isolated following the appropriate protocols outlined in the manual.

Important:

1. If automating this procedure on a liquid handler or a magnetic processor, please contact your Omega Bio-tek representative for instrument-specific instructions.
2. This kit includes enough reagents for the specified number of preparations plus at least an additional 10% overage to ensure there is sufficient volume. Please be aware that the actual number of preparations may be lower due to pre-aliquoting
of reagents, processing partial plates, and automation platform used etc. Additional reagents are available for purchase separately.