E.Z.N.A.® Plasmid DNA Mini Kit I, (V-spin)

Price range: $0.00 through $524.60

Rapid – Purification of plasmid DNA in less than 30 minutes
Safe – No Phenol/chloroform extractions
Versatile – Spin and vacuum formats available
High-quality – DNA is suitable for a variety of downstream applications

Size

Sample
5 Preps
50 Preps
200 Preps
600 Preps

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E.Z.N.A.® Plasmid DNA Mini Kit I, (V-spin)

The E.Z.N.A.® Plasmid DNA Mini Kit I is designed to isolate up to 25 µg of high-quality plasmid DNA from 1-5 mL bacterial cultures in less than 30 minutes. Plasmid DNA purification follows the alkaline-lysis method and is simplified with HiBind® Mini Column technology into three quick steps: Bind, Wash, and Elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing.

The E.Z.N.A.® Plasmid DNA Mini Kit is available in two formats: Q-spin (D6942) and V-spin (D6943). D6942 (Q-spin) features columns that are capless, whereas D6943 (V-spin) includes columns that have a cap attached. The columns are otherwise identical in use and application and can be used in both vacuum or centrifugation protocols.

For Research Use Only. Not for use in diagnostic procedures.

FEATURES	SPECIFICATIONS
Downstream Application	Cloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc.
Starting material	1-5 mL LB culture
Plasmid type	High-copy, low-copy, cosmid DNA
Processing mode	Manual (centrifugation or vacuum)
Throughput	1-24
DNA binding technology	Silica Mini Spin Column
Lysate clearance method	Centrifugation
Processing time	<30 minutes
Yield	15-25 µg for high copy-number; 0.1-5 µg for low copy-number
Special note	The alternative for discontinued yeast plasmid kit

ITEM	AVAILABLE SEPARATELY
HiBind® DNA Mini Columns	View Product
2 mL Collection Tubes	View Product
Solution I	View Product
Solution II	View Product
Solution III	View Product
HBC Buffer	View Product
DNA Wash Buffer	View Product
RNase A	View Product
Elution Buffer	View Product

High-Quality Plasmid Extractions

Figure 1. pGEM and pGL4.51 plasmids were purified from 5 mL DH5α cultures and eluted in 100 µL using Omega Bio-tek’s E.Z.N.A.® Plasmid DNA Mini Kit I. Plasmid DNA concentrations were determined by optical density measurements using Thermo Scientific’s NanoDrop™ 2000c system. All samples have ideal A260/A280 (~1.8) and A260/A230 (~2.0) absorbance ratios, indicating high-quality purified DNA.

Unfragmented Plasmid Purification

Figure 2. 400 ng purified pGEM and pGL4.51 plasmid were analyzed on a 0.8% Agarose gel. Plasmids were isolated from 5 mL DH5α cultures using Omega Bio-tek’s E.Z.N.A.® Plasmid DNA Mini Kit I. Plasmids were digested with HF-Scal to linearize.

Sequencing-Quality Plasmid Purification

Plasmid Type	Sample	Trace Score	Continuous Read Length	QV20+
pGEM	1	54	955	958
	2	53	965	965
	3	56	963	967
pGL4.51	1	56	977	978
	2	56	960	963
	3	56	962	960

Table 1. pGEM and pGL4.51 plasmids were purified from 5 mL DH5α cultures and eluted in 100 µL using Omega Bio-tek’s E.Z.N.A.® Plasmid DNA Mini Kit I. The plasmids were then used for Sanger Sequencing. DNA was analyzed on an Applied Biosystems 3730XL. All samples display high continuous read length and QV20+ scores, indicating plasmids of high-quality isolated from this kit.

Product Information

Document Name	Languages	Link
E.Z.N.A® Plasmid DNA Mini Kit I	English	Download
Protocol Manual	English	Download
Quick Guide	English	Download

Safety Data Sheets

Components	Hazard Standards	Languages	Link	hf:tax:dlp_document_language	hf:tax:dlp_document_hazard-standard
Solution I	REACH	Swedish	Download	swedish	reach
Solution I	GHS	English	Download	english	ghs
Solution I	REACH	Latvian	Download	latvian	reach
Solution I	REACH	Polish	Download	polish	reach
Solution I	WHMS	French	Download	french	whms
Solution I	WHMS	English	Download	english	whms
Solution I	REACH	Spanish	Download	spanish	reach
Solution I	REACH	Norwegian	Download	norwegian	reach
Solution I	REACH	Italian	Download	italian	reach
Solution I	REACH	German	Download	german	reach
Solution I	REACH	French	Download	french	reach
Solution I	REACH	Finnish	Download	finnish	reach
Solution I	REACH	Danish	Download	danish	reach
Solution I	REACH	English	Download	english	reach
Solution I	GHS	Spanish	Download	spanish	ghs
Solution II	REACH	Spanish	Download	spanish	reach
Solution II	REACH	Polish	Download	polish	reach
Solution II	REACH	Latvian	Download	latvian	reach
Solution II	WHMS	French	Download	french	whms
Solution II	WHMS	English	Download	english	whms
Solution II	REACH	Swedish	Download	swedish	reach
Solution II	REACH	Italian	Download	italian	reach
Solution II	REACH	German	Download	german	reach
Solution II	REACH	French	Download	french	reach
Solution II	REACH	Finnish	Download	finnish	reach
Solution II	REACH	Norwegian	Download	norwegian	reach
Solution II	GHS	English	Download	english	ghs
Solution II	GHS	Spanish	Download	spanish	ghs
Solution II	REACH	English	Download	english	reach
Solution II	REACH	Danish	Download	danish	reach
Solution III	REACH	Polish	Download	polish	reach
Solution III	REACH	Latvian	Download	latvian	reach
Solution III	WHMS	French	Download	french	whms
Solution III	WHMS	English	Download	english	whms
Solution III	REACH	Swedish	Download	swedish	reach
Solution III	REACH	Spanish	Download	spanish	reach
Solution III	REACH	Norwegian	Download	norwegian	reach
Solution III	REACH	Italian	Download	italian	reach
Solution III	REACH	German	Download	german	reach
Solution III	REACH	French	Download	french	reach
Solution III	REACH	Finnish	Download	finnish	reach
Solution III	REACH	Danish	Download	danish	reach
Solution III	REACH	English	Download	english	reach
Solution III	GHS	Spanish	Download	spanish	ghs
Solution III	GHS	English	Download	english	ghs