The E.Z.N.A.® Micro RNA Kit combines the reversible binding properties of the HiBind® matrix, a new silica-based material, with the highly efficient lysis ability of RNA-Solv® to extract micro and large (>200 nt) RNA from a wide variety of starting materials. Micro and large RNA are separated to allow for quantification into downstream applications.
The system uses RNA-Solv® to efficiently lyse cells and protect RNA from degradation. Following a chloroform separation step, RNA is applied to a HiBind® RNA column where large RNA binds. The flow-through is retained and applied to a HiBind® Micro RNA column to bind small RNA.
For Research Use Only. Not for use in diagnostic procedures.
FEATURES | SPECIFICATIONS |
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Downstream Application | RTPCR*, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation |
Elution Volume | 15-30 μL for miRNA; 40-70 μL for >200 nt |
Starting Material | Cultured eukaryotic cells or bacteria, or from animal, plant, or fungal tissues |
Starting Amount | Up to 100 mg |
RNA Yield | Up to 50 µg |
Processing Mode | Spin column |
Throughput | 1 – 24 |
RNA Binding Technology | Silica technology |
Binding Capacity | 100 µg for each |
ITEM | AVAILABLE SEPARATELY |
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HiBind® Micro RNA Column | — |
HiBind® RNA Mini Column | — |
2 mL Collection Tubes | View Product |
RNA-Solv® | View Product |
RNA Wash Buffer I | View Product |
RNA Wash Buffer II | View Product |
Nuclease-free Water | — |
Figure 1. Purified large RNAs and miRNA from tissue samples was isolated with the E.Z.N.A.® miRNA Isolation Kit. RNA was extracted from samples as follows: sample 1 from fish liver, sample 2 from frog liver. Large RNA (5% of total purified RNA) was analyzed on a 1% agarose gel. miRNA (5% of total purified RNA) was analyzed on a 3% agarose gel to demonstrate yield and quality of the RNA.
Product Manuals
Product Information
Document Name | Languages | Link |
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Safety Data Sheets
Components | Hazard Standards | Languages | Link | hf:tax:dlp_document_language | hf:tax:dlp_document_hazard-standard |
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Nuclease-free Water | GHS | Spanish | spanish | ghs | |
Nuclease-free Water | REACH | Greek | greek | reach | |
Nuclease-free Water | REACH | Portuguese | portuguese | reach | |
Nuclease-free Water | REACH | Hungarian | hungarian | reach | |
Nuclease-free Water | REACH | Dutch | dutch | reach | |
Nuclease-free Water | WHMS | French | french | whms | |
Nuclease-free Water | WHMS | English | english | whms | |
Nuclease-free Water | REACH | Swedish | swedish | reach | |
Nuclease-free Water | REACH | Spanish | spanish | reach | |
Nuclease-free Water | REACH | Italian | italian | reach | |
Nuclease-free Water | GHS | English | english | ghs | |
Nuclease-free Water | REACH | English | english | reach | |
Nuclease-free Water | REACH | Danish | danish | reach | |
Nuclease-free Water | REACH | French | french | reach | |
Nuclease-free Water | REACH | Norwegian | norwegian | reach | |
Nuclease-free Water | REACH | German | german | reach | |
Nuclease-free Water | REACH | Finnish | finnish | reach | |
RNA Solv Reagent | GHS | English | english | ghs | |
RNA Solv Reagent | WHMS | French | french | whms | |
RNA Solv Reagent | WHMS | English | english | whms | |
RNA Solv Reagent | REACH | Swedish | swedish | reach | |
RNA Solv Reagent | REACH | Spanish | spanish | reach | |
RNA Solv Reagent | REACH | Norwegian | norwegian | reach | |
RNA Solv Reagent | REACH | German | german | reach | |
RNA Solv Reagent | GHS | Spanish | spanish | ghs | |
RNA Solv Reagent | REACH | English | english | reach | |
RNA Solv Reagent | REACH | Danish | danish | reach | |
RNA Solv Reagent | REACH | French | french | reach | |
RNA Solv Reagent | REACH | Italian | italian | reach | |
RNA Solv Reagent | REACH | Finnish | finnish | reach | |
RNA Wash Buffer I | GHS | Spanish | spanish | ghs | |
RNA Wash Buffer I | WHMS | French | french | whms | |
RNA Wash Buffer I | WHMS | English | english | whms | |
RNA Wash Buffer I | REACH | Swedish | swedish | reach | |
RNA Wash Buffer I | REACH | Spanish | spanish | reach | |
RNA Wash Buffer I | REACH | Norwegian | norwegian | reach | |
RNA Wash Buffer I | REACH | German | german | reach | |
RNA Wash Buffer I | GHS | English | english | ghs | |
RNA Wash Buffer I | REACH | English | english | reach | |
RNA Wash Buffer I | REACH | Danish | danish | reach | |
RNA Wash Buffer I | REACH | Finnish | finnish | reach | |
RNA Wash Buffer I | REACH | French | french | reach | |
RNA Wash Buffer I | REACH | Italian | italian | reach | |
RNA Wash Buffer II | GHS | English | english | ghs | |
RNA Wash Buffer II | GHS | Spanish | spanish | ghs | |
RNA Wash Buffer II | REACH | English | english | reach | |
RNA Wash Buffer II | REACH | Danish | danish | reach | |
RNA Wash Buffer II | REACH | Finnish | finnish | reach | |
RNA Wash Buffer II | REACH | French | french | reach | |
RNA Wash Buffer II | REACH | German | german | reach | |
RNA Wash Buffer II | REACH | Italian | italian | reach | |
RNA Wash Buffer II | REACH | Norwegian | norwegian | reach | |
RNA Wash Buffer II | REACH | Spanish | spanish | reach | |
RNA Wash Buffer II | REACH | Swedish | swedish | reach | |
RNA Wash Buffer II | WHMS | English | english | whms | |
RNA Wash Buffer II | WHMS | French | french | whms |