Mag-Bind® Endo-free Plasmid Mini Kit

$0.00$630.00

Purify endotoxin free (<0.1 EU/µg) plasmid DNA using magnetic beads from up to 1.5 mL culture volume.

  • Fully Automatable – Magnetic beads clear the lysate, avoiding the manual centrifugation step
  • High-yielding – Up to 20 μg plasmid DNA from 1.5 mL TB culture
  • Versatile – Process up to 1.5 mL of different culture medias with different plasmid
  • Transfection-quality – Endotoxin-free plasmid DNA with endotoxins levels < 0.1 EU/µg
  • Pure – Contamination-free plasmid DNA with A260/A280 > 1.8 and A260/A230 > 1.8

Included with each kit:

Ready-to-implement automated purification solution

All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.

Off-the-shelf product doesn’t suit your needs? We’ll work with you to develop a customized product. Learn more.

Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications. Learn more.

The Mag-Bind® Endo-free Plasmid Mini Kit is designed to deliver high-quality, plasmid DNA with endotoxin levels <0.1 EU/μg for use in eukaryotic transfections and other sensitive in vitro applications.

The kit follows magnetic bead-based technology to purify plasmid DNA from up to 1.5 mL overnight bacterial culture in LB or TB or other suitable growth medium. The Mag-Bind® Endo-free Plasmid Mini Kit can be processed either manually or automated on most open-ended liquid handling platforms as well as magnetic processors. Purified plasmid DNA is endotoxin free (<0.1 EU/μg), and yields may vary depending on the copy number of the plasmid, E. coli strain used, culture media, and conditions of growth.


The Mag-Bind® Endo-free Plasmid Mini Kit follows a modified alkaline lysis method forming a bacterial lysate. The lysate is then cleared using either Mag-Bind® Particles LC (sold separately) or centrifugation. The novel paramagnetic lysate clearance beads bind to cell debris and pulls them down when magnetized. This eliminates the need for centrifugation or vacuum manifold for lysate clearance, making this suitable for high-throughput, automation workflows.

Post lysate clearance, plasmid DNA is then bound to Mag-Bind® Particles RQ through a specially formulated binding buffer and the Mag-Bind® particles are subjected to a wash routine to remove endotoxins, salt, and other contaminants. High-quality plasmid DNA is then eluted in Endo-free Water and is ready for use in a wide range of downstream applications such as transfection, routine screening, restriction enzyme digestion, transformation, PCR, and DNA sequencing etc.

For Research Use Only. Not for use in diagnostic procedures.

Feature Specifications
Starting Amount up to 1.5 mL overnight bacterial culture per well of a 96-well deep well plate
Starting Material Bacteria harboring high-copy or low-copy plasmid in LB, TB, or other suitable culture medium
Elution Volume 50 - 100 µL
Technology Magnetic beads
Processing Mode Automated, Manual
Throughput 1 - 96
Processing Time 96 samples in 95 minutes
Endotoxin levels < 0.1 EU/µg
Purity Contamination-free plasmid DNA with A260/A280 > 1.8 and A260/A230 > 1.8
Lysate Clearance Methodology Magnetic beads or Centrifugation
Downstream Applications Transfection, routine screening, restriction enzyme digestion, transformation, PCR, DNA sequencing, in vitro transcription, and other sensitive applications requiring pure DNA
SKU No M1261-00 M1261-01 Available Seperately
Purifications 1 x 96 4 x 96
Solution I 30 mL 120 mL Solution I
Solution II 30 mL 120 mL Solution II
N3 Buffer 40 mL 85 mL
IRD Buffer 50 mL 200 mL IRD Buffer
VHB Buffer 66 mL 264 mL VHB Buffer
Endo-free Water 15 mL 60 mL
Mag-Bind® Particles RQ 2.2 mL 8.8 mL Mag-Bind® Particles RQ
RNase A 100 μL 400 μL RNase A

Components for optional protocol:

Product Literature

NameDocumentLanguagesLink
Protocol ManualProduct ManualEnglish
Mag-Bind Endo-free Plasmid Mini KitProduct LiteratureEnglish
High-throughput, Automated Plasmid Purification from up to 1.5 mL Bacterial CulturesApplication NoteEnglish
Automated Plasmid Purifications using the MagBinder Fit24: Mini, Midi, and MoreApplication NoteEnglish

~1.8X higher plasmid yields and lower endotoxin levels

Table 1. pGEM plasmid DNA was purified from 1.5 mL DH5α cultures (n=3) using the Mag-Bind® Endo-free Plasmid Mini Kit, as well as Company P’s Kit, following manufacturers’ recommended protocol. Purified Plasmid DNA was quantified using Thermo Scientific’s NanoDrop™ 2000c system. Plasmid preparation was not only endotoxin-free (<0.1 EU/μg) for Omega Bio-tek’s Kit, but the yield was also significantly higher compared to Company P’s Kit.

Higher transfection efficiency vs Company P

Figure 1. pGEM plasmid was prepared from 1.5 mL DH5α cultures (n=3) and purified using the Mag-Bind® Endo-free Plasmid Mini Kit, as well as Company P’s equivalent Kit. Purified Plasmids were transfected into 10,000 cells with 200 ng of purified plasmid using 2 μL Lipofectamine Transfection Reagent. Plasmids purified using Omega Bio-tek’s Kit resulted in higher bioluminscent signal, indicating higher transfection efficiency compared to plasmids purified using Company P’s Kit.

Consistent yields and purity of Plasmid preps automated on Hamilton Microlab® Star™

Figure 2. pGEM plasmid was purified from 1 mL DH5α cultures using the Mag-Bind® Endo-free Mini Kit, fully automated on the Hamilton Microlab® STAR™ platform, starting from a bacterial pellet and using lysate clearance beads. Purified plasmid DNA was eluted in 100 μL and quantified using Thermo Scientific’s NanoDrop™ 2000c system. Plasmid yields were consistent among the replicates and had excellent purity ratios with A260/A280 ~1.8 and A260/A230 ~2.0.

High Yield Across Various Plasmid Types and Culture Media

Figure 3. Mag-Bind® Endo-free Plasmid Mini Kit is capable of handling diverse sample input conditions ranging from plasmid types, plasmid sizes, culture medium, and input culture volumes. The plasmid yield may vary based on these conditions.

Product Literature

NameDocumentLanguagesLink
Protocol ManualProduct ManualEnglish
Mag-Bind Endo-free Plasmid Mini KitProduct LiteratureEnglish
High-throughput, Automated Plasmid Purification from up to 1.5 mL Bacterial CulturesApplication NoteEnglish
Automated Plasmid Purifications using the MagBinder Fit24: Mini, Midi, and MoreApplication NoteEnglish

Product Information

NameDocumentLanguagesLink
Protocol ManualProduct ManualEnglish
Mag-Bind Endo-free Plasmid Mini KitProduct LiteratureEnglish
High-throughput, Automated Plasmid Purification from up to 1.5 mL Bacterial CulturesApplication NoteEnglish
Automated Plasmid Purifications using the MagBinder Fit24: Mini, Midi, and MoreApplication NoteEnglish

Safety Data Sheets

ComponentsHazard StandardsLanguagesLinkhf:tax:dlp_document_languagehf:tax:dlp_document_hazard-standard
Endo-free WaterGHSEnglishenglishghs
Endo-free WaterGHSSpanishspanishghs
Endo-free WaterREACHEnglishenglishreach
Endo-free WaterWHMSEnglishenglishwhms
Endo-free WaterWHMSFrenchfrenchwhms
Endo-free WaterREACHDanishdanishreach
Endo-free WaterREACHDutchdutchreach
Endo-free WaterREACHFinnishfinnishreach
Endo-free WaterREACHFrenchfrenchreach
Endo-free WaterREACHGermangermanreach
Endo-free WaterREACHGreekgreekreach
Endo-free WaterREACHHungarianhungarianreach
Endo-free WaterREACHItalianitalianreach
Endo-free WaterREACHNorwegiannorwegianreach
Endo-free WaterREACHPortugueseportuguesereach
Endo-free WaterREACHSpanishspanishreach
Endo-free WaterREACHSwedishswedishreach
IRD BufferGHSEnglishenglishghs
IRD BufferGHSSpanishspanishghs
IRD BufferREACHEnglishenglishreach
IRD BufferWHMSEnglishenglishwhms
IRD BufferWHMSFrenchfrenchwhms
IRD BufferREACHDanishdanishreach
IRD BufferREACHDutchdutchreach
IRD BufferREACHFinnishfinnishreach
IRD BufferREACHFrenchfrenchreach
IRD BufferREACHGermangermanreach
IRD BufferREACHGreekgreekreach
IRD BufferREACHHungarianhungarianreach
IRD BufferREACHItalianitalianreach
IRD BufferREACHNorwegiannorwegianreach
IRD BufferREACHPortugueseportuguesereach
IRD BufferREACHSpanishspanishreach
IRD BufferREACHSwedishswedishreach
Mag-Bind Particles RQGHSEnglishenglishghs
Mag-Bind Particles RQGHSSpanishspanishghs
Mag-Bind Particles RQREACHEnglishenglishreach
Mag-Bind Particles RQREACHDanishdanishreach
Mag-Bind Particles RQREACHFinnishfinnishreach
Mag-Bind Particles RQREACHFrenchfrenchreach
Mag-Bind Particles RQREACHGermangermanreach
Mag-Bind Particles RQREACHItalianitalianreach
Mag-Bind Particles RQREACHNorwegiannorwegianreach
Mag-Bind Particles RQREACHSpanishspanishreach
Mag-Bind Particles RQREACHSwedishswedishreach
Mag-Bind Particles RQWHMSEnglishenglishwhms
Mag-Bind Particles RQWHMSFrenchfrenchwhms
Mag-Bind Particles RQREACHDutchdutchreach
Mag-Bind Particles RQREACHHungarianhungarianreach
Mag-Bind Particles RQREACHPortugueseportuguesereach
Mag-Bind Particles RQREACHGreekgreekreach
N3 BufferGHSEnglishenglishghs
N3 BufferGHSSpanishspanishghs
N3 BufferREACHEnglishenglishreach
N3 BufferREACHDanishdanishreach
N3 BufferREACHFinnishfinnishreach
N3 BufferREACHFrenchfrenchreach
N3 BufferREACHGermangermanreach
N3 BufferREACHItalianitalianreach
N3 BufferREACHNorwegiannorwegianreach
N3 BufferREACHSpanishspanishreach
N3 BufferREACHSwedishswedishreach
N3 BufferWHMSEnglishenglishwhms
N3 BufferWHMSFrenchfrenchwhms
RNase AGHSEnglishenglishghs
RNase AGHSSpanishspanishghs
RNase AREACHEnglishenglishreach
RNase AREACHDanishdanishreach
RNase AREACHFinnishfinnishreach
RNase AREACHFrenchfrenchreach
RNase AREACHGermangermanreach
RNase AREACHItalianitalianreach
RNase AREACHNorwegiannorwegianreach
RNase AREACHSpanishspanishreach
RNase AREACHSwedishswedishreach
RNase AWHMSEnglishenglishwhms
RNase AWHMSFrenchfrenchwhms
Solution IGHSEnglishenglishghs
Solution IGHSSpanishspanishghs
Solution IREACHEnglishenglishreach
Solution IREACHDanishdanishreach
Solution IREACHFinnishfinnishreach
Solution IREACHFrenchfrenchreach
Solution IREACHGermangermanreach
Solution IREACHItalianitalianreach
Solution IREACHNorwegiannorwegianreach
Solution IREACHSpanishspanishreach
Solution IREACHSwedishswedishreach
Solution IWHMSEnglishenglishwhms
Solution IWHMSFrenchfrenchwhms
Solution IIGHSEnglishenglishghs
Solution IIGHSSpanishspanishghs
Solution IIREACHEnglishenglishreach
Solution IIREACHDanishdanishreach
Solution IIREACHFinnishfinnishreach
Solution IIREACHFrenchfrenchreach
Solution IIREACHGermangermanreach
Solution IIREACHItalianitalianreach
Solution IIREACHNorwegiannorwegianreach
Solution IIREACHSpanishspanishreach
Solution IIREACHSwedishswedishreach
Solution IIWHMSEnglishenglishwhms
Solution IIWHMSFrenchfrenchwhms
VHB BufferGHSEnglishenglishghs
VHB BufferGHSSpanishspanishghs
VHB BufferREACHEnglishenglishreach
VHB BufferREACHDanishdanishreach
VHB BufferREACHFinnishfinnishreach
VHB BufferREACHFrenchfrenchreach
VHB BufferREACHGermangermanreach
VHB BufferREACHItalianitalianreach
VHB BufferREACHNorwegiannorwegianreach
VHB BufferREACHSpanishspanishreach
VHB BufferREACHSwedishswedishreach
VHB BufferWHMSEnglishenglishwhms
VHB BufferWHMSFrenchfrenchwhms
VHB BufferREACHDutchdutchreach
VHB BufferREACHHungarianhungarianreach
VHB BufferREACHPortugueseportuguesereach
VHB BufferREACHGreekgreekreach
Shopping Cart
Scroll to Top