Using the Mag-Bind® Endo-free Plasmid Mini Kit

on the Hamilton Microlab® STAR™

The Mag-Bind® Endo-free Plasmid Mini Kit (M1261) extracts high-yielding, high-quality plasmid DNA from a wide range of plasmid types and culture media:

Fully Automatable – Eliminate manual centrifugation using Lysate Clearance Beads (available separately).
Transfection-quality – Endotoxin-free plasmid DNA with endotoxins levels < 0.1 EU/µg.
Pure – Contamination-free plasmid DNA with A260/A280 > 1.8 and A260/A230 > 1.8.

Download our technical note describing this protocol: High-throughput, Automated Plasmid Purification from up to 1.5 mL Bacterial Cultures

Automated Protocol Specifications

Feature	Specification
Sample Amount	1.5 mL LB or TB culture
Throughput	4 x 96 well-plates
Online Processing Time	95 minutes

Contact us to learn how to get this ready-to-run protocol and script:

M1261 on a Hamilton Liquid Handler Information Request

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Mag-Bind® Endo-free Plasmid Mini Kit

Purify endotoxin free (<0.1 EU/µg) plasmid DNA using magnetic beads from up to 1.5 mL culture volume.

Fully Automatable – Magnetic beads clear the lysate, avoiding the manual centrifugation step
High-yielding – Up to 20 μg plasmid DNA from 1.5 mL TB culture
Versatile – Process up to 1.5 mL of different culture medias with different plasmid
Transfection-quality – Endotoxin-free plasmid DNA with endotoxins levels < 0.1 EU/µg
Pure – Contamination-free plasmid DNA with A260/A280 > 1.8 and A260/A230 > 1.8

Learn more about the kit:

Mag-Bind® Endo-free Plasmid Mini Kit

Automated Solution Data

Consistent yields and purity of Plasmid preps automated on Hamilton Microlab® Star™

Figure 1. pGEM plasmid was purified from 1 mL DH5α cultures using the Mag-Bind® Endo-free Mini Kit, fully automated on the Hamilton Microlab® STAR™ platform, starting from a bacterial pellet and using lysate clearance beads. Purified plasmid DNA was eluted in 100 μL and quantified using Thermo Scientific’s NanoDrop™ 2000c system. Plasmid yields were consistent among the replicates and had excellent purity ratios with A260/A280 ~1.8 and A260/A230 ~2.0.

Ready-to-implement automated purification solution

All Mag-Bind® kits can be automated on most programmable robotic platforms, liquid handlers or magnetic processors. Learn more.

Off-the-shelf product doesn’t suit your needs? We’ll work with you to develop a customized product. Learn more.

Dedicated applications support will consult with you to develop and implement an automated solution that fits your specifications. Learn more.

Scripts available for all Mag-Bind® Kits for most open liquid handlers and common magnetic processors including the MagBinder® Fit²⁴. Visit Automated Solutions for more information.